Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 242443..243085 Replicon chromosome
Accession NZ_CP020743
Organism Bacillus mycoides strain Gnyt1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag B7492_RS01375 Protein ID WP_000635965.1
Coordinates 242735..243085 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag B7492_RS01370 Protein ID WP_000004570.1
Coordinates 242443..242730 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7492_RS01345 237759..238721 + 963 WP_002029436.1 UV DNA damage repair endonuclease UvsE -
B7492_RS01350 238714..239286 - 573 WP_002167128.1 rhomboid family intramembrane serine protease -
B7492_RS01355 239378..239737 + 360 WP_002009969.1 holo-ACP synthase -
B7492_RS01360 239894..240844 + 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
B7492_RS01365 240962..242131 + 1170 WP_085308684.1 alanine racemase -
B7492_RS01370 242443..242730 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
B7492_RS01375 242735..243085 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B7492_RS01380 243154..245322 + 2169 WP_085312924.1 RNA-binding transcriptional accessory protein -
B7492_RS01385 245380..245496 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
B7492_RS01390 245707..246162 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T75374 WP_000635965.1 NZ_CP020743:242735-243085 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T75374 NZ_CP020743:242735-243085 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT75374 WP_000004570.1 NZ_CP020743:242443-242730 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT75374 NZ_CP020743:242443-242730 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References