Detailed information of TA system TA075370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-pasA/PRK09812-HTH
• Location: 1031430..1032097
• Replicon: chromosome
• Accession: NZ_CP020742
• Organism: Bartonella henselae strain Houston-I

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: X5M710
• Locus tag: BhenCHDE101_RS04600
• Protein ID: WP_011180620.1
• Coordinates: 1031765..1032097 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: N6VJG4
• Locus tag: BhenCHDE101_RS04595
• Protein ID: WP_007346764.1
• Coordinates: 1031430..1031657 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BhenCHDE101_RS04565	1028270..1029595	-	1326	WP_011180823.1	phage terminase large subunit	-
BhenCHDE101_RS04570	1029576..1029902	-	327	WP_011180626.1	hypothetical protein	-
BhenCHDE101_RS04580	1030201..1030659	+	459	WP_011180624.1	hypothetical protein	-
BhenCHDE101_RS04590	1031177..1031446	-	270	WP_011180622.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BhenCHDE101_RS04595	1031430..1031657	-	228	WP_007346764.1	ribbon-helix-helix protein, CopG family	Antitoxin
BhenCHDE101_RS04600	1031765..1032097	-	333	WP_011180620.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BhenCHDE101_RS04605	1032097..1032354	-	258	WP_034448603.1	antitoxin	-
BhenCHDE101_RS04610	1032435..1032929	-	495	WP_059443338.1	hypothetical protein	-
BhenCHDE101_RS04615	1032983..1033429	-	447	WP_011180617.1	single-stranded DNA-binding protein	-
BhenCHDE101_RS04620	1033641..1033931	+	291	WP_011180616.1	hypothetical protein	-
BhenCHDE101_RS04625	1033928..1034200	+	273	WP_011180615.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
BhenCHDE101_RS04630	1034388..1034708	-	321	Protein_863	hypothetical protein	-
BhenCHDE101_RS04635	1034890..1035276	-	387	WP_082246570.1	hypothetical protein	-
BhenCHDE101_RS04640	1035260..1036510	-	1251	WP_011180612.1	DUF1376 domain-containing protein	-
BhenCHDE101_RS04645	1036500..1036979	-	480	WP_011180611.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1005272..1045394	40122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Protein of unknown function DUF6290 (2-71)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11865.88 Da
• Isoelectric Point: 10.5590

>T75370 WP_011180620.1 NZ_CP020742:c1032097-1031765 [Bartonella henselae]
MKRGEIWLVSLDPTSGYEQKGTRPVLIVSPEAFNRVTKTPIVLPITSGGNFSRTAGFAVSLVGTGLHTTGVIRCDQPRAL
DIAARQGKKMETVPPIIMNEVLAKLSTFLT

Nucleotide

• Length: 333 bp

>T75370 NZ_CP020742:c1032097-1031765 [Bartonella henselae]
ATGAAGCGGGGCGAAATTTGGCTTGTATCTCTTGACCCAACTTCAGGCTATGAACAAAAAGGAACGCGTCCTGTATTGAT
TGTATCACCAGAGGCGTTTAATCGTGTCACGAAAACACCCATTGTCTTACCCATTACAAGTGGAGGAAATTTTTCTCGAA
CCGCAGGTTTCGCTGTCTCATTGGTAGGAACAGGATTGCATACAACAGGTGTGATACGTTGTGACCAGCCACGTGCTCTG
GATATAGCAGCGCGTCAGGGTAAGAAAATGGAAACAGTTCCCCCCATAATTATGAATGAAGTTCTTGCTAAGCTATCAAC
ATTTCTCACATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8742.78 Da
• Isoelectric Point: 4.8780

>AT75370 WP_007346764.1 NZ_CP020742:c1031657-1031430 [Bartonella henselae]
MTISIRLPSDLETRLNNLAAKTGRTKSFYLREIIERGIEEAEDYYLASQVRERVRRGEGTFYSSEEVRKELGLDD

Nucleotide

• Length: 228 bp

>AT75370 NZ_CP020742:c1031657-1031430 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAGGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Structures

Toxin

• AlphaFold DB: A0A067W4K9

Antitoxin

• AlphaFold DB: N6VJG4