Detailed information of TA system TA075365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 2679642..2680750
• Replicon: chromosome
• Accession: NZ_CP020741
• Organism: Staphylococcus aureus strain HZW450

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: B7437_RS13750
• Protein ID: WP_000233000.1
• Coordinates: 2679642..2680511 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: B7437_RS13755
• Protein ID: WP_000205227.1
• Coordinates: 2680526..2680750 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7437_RS13715	2675135..2675872	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
B7437_RS13720	2675997..2676080	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
B7437_RS13730	2676622..2677416	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
B7437_RS13735	2677509..2677989	-	481	Protein_2557	GNAT family N-acetyltransferase	-
B7437_RS13740	2677986..2678894	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
B7437_RS13745	2678927..2679661	-	735	WP_096827996.1	class I SAM-dependent methyltransferase	-
B7437_RS13750	2679642..2680511	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
B7437_RS13755	2680526..2680750	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
B7437_RS13760	2680893..2682437	-	1545	WP_002390960.1	recombinase family protein	-
B7437_RS13765	2682457..2682873	-	417	WP_000323438.1	recombinase	-
B7437_RS13770	2682874..2683170	-	297	WP_002360685.1	hypothetical protein	-
B7437_RS13780	2683682..2684359	-	678	Protein_2565	DNA topoisomerase III	-
B7437_RS13785	2684375..2684977	-	603	WP_001062586.1	recombinase family protein	-
B7437_RS14670	2684991..2685161	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	erm(B) / aph(3')-III / ant(6)-Ia	-	2670219..2678894	8675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T75365 WP_000233000.1 NZ_CP020741:c2680511-2679642 [Staphylococcus aureus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T75365 NZ_CP020741:c2680511-2679642 [Staphylococcus aureus]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT75365 WP_000205227.1 NZ_CP020741:c2680750-2680526 [Staphylococcus aureus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT75365 NZ_CP020741:c2680750-2680526 [Staphylococcus aureus]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1