Detailed information of TA system TA075360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2115271..2115800
• Replicon: chromosome
• Accession: NZ_CP020741
• Organism: Staphylococcus aureus strain HZW450

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B7437_RS10795
• Protein ID: WP_000621175.1
• Coordinates: 2115271..2115633 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: B7437_RS10800
• Protein ID: WP_000948331.1
• Coordinates: 2115630..2115800 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7437_RS10770	2112249..2113019	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
B7437_RS10775	2112994..2113473	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
B7437_RS10780	2113475..2113801	-	327	WP_001052491.1	anti-sigma factor antagonist	-
B7437_RS10785	2113920..2114921	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
B7437_RS10795	2115271..2115633	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7437_RS10800	2115630..2115800	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
B7437_RS10805	2115885..2117033	-	1149	WP_001280702.1	alanine racemase	-
B7437_RS10810	2117099..2117458	-	360	WP_000581194.1	holo-ACP synthase	-
B7437_RS10815	2117462..2117953	-	492	WP_001205915.1	PH domain-containing protein	-
B7437_RS10820	2117940..2119523	-	1584	WP_001294642.1	PH domain-containing protein	-
B7437_RS10825	2119516..2119995	-	480	WP_001287083.1	hypothetical protein	-
B7437_RS10830	2120204..2120764	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T75360 WP_000621175.1 NZ_CP020741:c2115633-2115271 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T75360 NZ_CP020741:c2115633-2115271 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT75360 WP_000948331.1 NZ_CP020741:c2115800-2115630 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT75360 NZ_CP020741:c2115800-2115630 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1