Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2384778..2385753 Replicon chromosome
Accession NZ_CP020723
Organism Bacillus thuringiensis strain BM-BT15426

Toxin (Protein)


Gene name spoIISA Uniprot ID C3GJ28
Locus tag B7P25_RS12805 Protein ID WP_003297560.1
Coordinates 2384778..2385515 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag B7P25_RS12810 Protein ID WP_000588712.1
Coordinates 2385628..2385753 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7P25_RS12775 2380324..2380731 + 408 WP_087931797.1 VOC family protein -
B7P25_RS12780 2380978..2381288 + 311 Protein_2325 hypothetical protein -
B7P25_RS12785 2381410..2383095 - 1686 WP_087931798.1 alpha-keto acid decarboxylase family protein -
B7P25_RS12790 2383202..2383684 + 483 WP_000191911.1 MarR family winged helix-turn-helix transcriptional regulator -
B7P25_RS12795 2383852..2384589 + 738 WP_000594156.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
B7P25_RS12805 2384778..2385515 + 738 WP_003297560.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
B7P25_RS12810 2385628..2385753 + 126 WP_000588712.1 hypothetical protein Antitoxin
B7P25_RS28940 2385830..2386006 + 177 WP_000808045.1 hypothetical protein -
B7P25_RS12820 2386150..2387619 + 1470 WP_087931800.1 beta-Ala-His dipeptidase -
B7P25_RS12830 2388201..2388671 - 471 WP_087931801.1 DUF5065 family protein -
B7P25_RS12835 2388848..2389468 + 621 WP_151909249.1 SGNH/GDSL hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28373.07 Da        Isoelectric Point: 8.2672

>T75348 WP_003297560.1 NZ_CP020723:2384778-2385515 [Bacillus thuringiensis]
VISNIRIGLFILAIVFLILVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T75348 NZ_CP020723:2384778-2385515 [Bacillus thuringiensis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTCATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT75348 WP_000588712.1 NZ_CP020723:2385628-2385753 [Bacillus thuringiensis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT75348 NZ_CP020723:2385628-2385753 [Bacillus thuringiensis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9EK80

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References