75347

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 251448..252090 Replicon chromosome
Accession NZ_CP020723
Organism Bacillus thuringiensis strain BM-BT15426

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag B7P25_RS01460 Protein ID WP_000635963.1
Coordinates 251740..252090 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag B7P25_RS01455 Protein ID WP_000004570.1
Coordinates 251448..251735 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7P25_RS01430 246764..247726 + 963 WP_087931185.1 UV DNA damage repair endonuclease UvsE -
B7P25_RS01435 247719..248291 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
B7P25_RS01440 248384..248743 + 360 WP_000635040.1 holo-ACP synthase -
B7P25_RS01445 248900..249850 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
B7P25_RS01450 249969..251138 + 1170 WP_000390600.1 alanine racemase -
B7P25_RS01455 251448..251735 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
B7P25_RS01460 251740..252090 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B7P25_RS01465 252158..254326 + 2169 WP_151909163.1 RNA-binding transcriptional accessory protein -
B7P25_RS01470 254383..254499 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
B7P25_RS01475 254695..255153 + 459 WP_087931187.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T75347 WP_000635963.1 NZ_CP020723:251740-252090 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T75347 NZ_CP020723:251740-252090 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT75347 WP_000004570.1 NZ_CP020723:251448-251735 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT75347 NZ_CP020723:251448-251735 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References