Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2685059..2685695 Replicon chromosome
Accession NZ_CP020722
Organism Bacillus subtilis strain Bacillus subtilis Bs-115

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag B7470_RS14260 Protein ID WP_003156187.1
Coordinates 2685345..2685695 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag B7470_RS14255 Protein ID WP_003225183.1
Coordinates 2685059..2685340 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7470_RS14235 2681418..2682017 - 600 WP_014478896.1 rhomboid family intramembrane serine protease -
B7470_RS14240 2682112..2682477 + 366 WP_014478897.1 holo-ACP synthase -
B7470_RS14245 2682643..2683659 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
B7470_RS14250 2683774..2684943 + 1170 WP_014478898.1 alanine racemase -
B7470_RS14255 2685059..2685340 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
B7470_RS14260 2685345..2685695 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B7470_RS14265 2685811..2686635 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
B7470_RS14270 2686640..2687005 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
B7470_RS14275 2687009..2687410 + 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
B7470_RS14280 2687422..2688429 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
B7470_RS14285 2688498..2688827 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
B7470_RS14290 2688824..2689306 + 483 WP_003234299.1 anti-sigma B factor RsbW -
B7470_RS14295 2689272..2690060 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
B7470_RS14300 2690060..2690659 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T75345 WP_003156187.1 NZ_CP020722:2685345-2685695 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T75345 NZ_CP020722:2685345-2685695 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT75345 WP_003225183.1 NZ_CP020722:2685059-2685340 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT75345 NZ_CP020722:2685059-2685340 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References