Detailed information of TA system TA075334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2225215..2225744
• Replicon: chromosome
• Accession: NZ_CP020714
• Organism: Staphylococcus aureus strain K18

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B7474_RS11620
• Protein ID: WP_000621175.1
• Coordinates: 2225215..2225577 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: B7474_RS11625
• Protein ID: WP_000948331.1
• Coordinates: 2225574..2225744 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7474_RS11595	2222193..2222963	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
B7474_RS11600	2222938..2223417	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
B7474_RS11605	2223419..2223745	-	327	WP_001052491.1	anti-sigma factor antagonist	-
B7474_RS11610	2223864..2224865	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
B7474_RS11620	2225215..2225577	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7474_RS11625	2225574..2225744	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
B7474_RS11630	2225829..2226977	-	1149	WP_001281154.1	alanine racemase	-
B7474_RS11635	2227043..2227402	-	360	WP_101766691.1	holo-ACP synthase	-
B7474_RS11640	2227406..2227897	-	492	WP_072353916.1	PH domain-containing protein	-
B7474_RS11645	2227884..2229467	-	1584	WP_001294626.1	PH domain-containing protein	-
B7474_RS11650	2229460..2229939	-	480	WP_031590204.1	hypothetical protein	-
B7474_RS11655	2230148..2230708	-	561	WP_101766690.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T75334 WP_000621175.1 NZ_CP020714:c2225577-2225215 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T75334 NZ_CP020714:c2225577-2225215 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT75334 WP_000948331.1 NZ_CP020714:c2225744-2225574 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT75334 NZ_CP020714:c2225744-2225574 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1