Detailed information of TA system TA075325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2604142..2604674
• Replicon: chromosome
• Accession: NZ_CP020713
• Organism: Staphylococcus aureus strain K17

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: Q8VLW9
• Locus tag: B7473_RS13515
• Protein ID: WP_001103944.1
• Coordinates: 2604354..2604674 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: B7473_RS13510
• Protein ID: WP_001058486.1
• Coordinates: 2604142..2604351 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7473_RS13475	2599388..2599672	+	285	WP_000917289.1	co-chaperone GroES	-
B7473_RS13480	2599748..2601364	+	1617	WP_000240642.1	chaperonin GroEL	-
B7473_RS13485	2601433..2602605	-	1173	WP_000179346.1	site-specific integrase	-
B7473_RS13490	2602619..2603293	-	675	WP_000620857.1	helix-turn-helix transcriptional regulator	-
B7473_RS13495	2603466..2603684	+	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
B7473_RS13500	2603689..2604006	+	318	WP_069724350.1	helix-turn-helix domain-containing protein	-
B7473_RS13505	2604003..2604149	+	147	WP_000708434.1	hypothetical protein	-
B7473_RS13510	2604142..2604351	+	210	WP_001058486.1	hypothetical protein	Antitoxin
B7473_RS13515	2604354..2604674	+	321	WP_001103944.1	DUF1474 family protein	Toxin
B7473_RS13520	2604741..2605610	+	870	WP_001002689.1	primase alpha helix C-terminal domain-containing protein	-
B7473_RS13525	2605624..2607324	+	1701	WP_000447470.1	DUF927 domain-containing protein	-
B7473_RS13530	2607654..2608034	+	381	WP_020977426.1	hypothetical protein	-
B7473_RS13535	2608031..2608672	+	642	WP_101766726.1	pathogenicity island protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	2596443..2608672	12229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12636.31 Da
• Isoelectric Point: 4.8519

>T75325 WP_001103944.1 NZ_CP020713:2604354-2604674 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLQVYLKEFDELIQKF
HEIEKASSDVSLATESDDAKKLKITE

Nucleotide

• Length: 321 bp

>T75325 NZ_CP020713:2604354-2604674 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTA
A

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8252.31 Da
• Isoelectric Point: 4.3718

>AT75325 WP_001058486.1 NZ_CP020713:2604142-2604351 [Staphylococcus aureus]
MNNEQKEVIEHLVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT75325 NZ_CP020713:2604142-2604351 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: Q8VLW9