Detailed information of TA system TA075322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2219939..2220468
• Replicon: chromosome
• Accession: NZ_CP020713
• Organism: Staphylococcus aureus strain K17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B7473_RS11455
• Protein ID: WP_000621175.1
• Coordinates: 2220106..2220468 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: B7473_RS11450
• Protein ID: WP_000948331.1
• Coordinates: 2219939..2220109 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7473_RS11420	2214975..2215535	+	561	WP_101766690.1	K(+)-transporting ATPase subunit C	-
B7473_RS11425	2215744..2216223	+	480	WP_031590204.1	hypothetical protein	-
B7473_RS11430	2216216..2217799	+	1584	WP_001294626.1	PH domain-containing protein	-
B7473_RS11435	2217786..2218277	+	492	WP_072353916.1	PH domain-containing protein	-
B7473_RS11440	2218281..2218640	+	360	WP_101766691.1	holo-ACP synthase	-
B7473_RS11445	2218706..2219854	+	1149	WP_001281154.1	alanine racemase	-
B7473_RS11450	2219939..2220109	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
B7473_RS11455	2220106..2220468	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7473_RS11465	2220818..2221819	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
B7473_RS11470	2221938..2222264	+	327	WP_001052491.1	anti-sigma factor antagonist	-
B7473_RS11475	2222266..2222745	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
B7473_RS11480	2222720..2223490	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T75322 WP_000621175.1 NZ_CP020713:2220106-2220468 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T75322 NZ_CP020713:2220106-2220468 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT75322 WP_000948331.1 NZ_CP020713:2219939-2220109 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT75322 NZ_CP020713:2219939-2220109 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1