Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 5370897..5371505 Replicon chromosome
Accession NZ_CP020704
Organism Pseudomonas aeruginosa strain PASGNDM699

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag B7W87_RS26130 Protein ID WP_003114156.1
Coordinates 5370897..5371244 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag B7W87_RS26135 Protein ID WP_003114155.1
Coordinates 5371254..5371505 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7W87_RS26100 5366256..5366528 + 273 WP_003134390.1 cysteine-rich CWC family protein -
B7W87_RS26105 5366528..5367220 + 693 WP_003085458.1 16S rRNA pseudouridine(516) synthase -
B7W87_RS26110 5367356..5368399 + 1044 WP_003134392.1 L,D-transpeptidase -
B7W87_RS26115 5368479..5369216 + 738 WP_003124252.1 murein L,D-transpeptidase catalytic domain family protein -
B7W87_RS26120 5369668..5370570 + 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
B7W87_RS26130 5370897..5371244 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B7W87_RS26135 5371254..5371505 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B7W87_RS26140 5371719..5372701 - 983 Protein_5044 tyrosine-type recombinase/integrase -
B7W87_RS26145 5372701..5373993 - 1293 WP_003134394.1 hypothetical protein -
B7W87_RS26150 5374223..5375506 - 1284 WP_023127773.1 hypothetical protein -
B7W87_RS26155 5375510..5375866 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5370897..5388599 17702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T75312 WP_003114156.1 NZ_CP020704:c5371244-5370897 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T75312 NZ_CP020704:c5371244-5370897 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT75312 WP_003114155.1 NZ_CP020704:c5371505-5371254 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT75312 NZ_CP020704:c5371505-5371254 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References