75300

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4885117..4885769 Replicon chromosome
Accession NZ_CP020703
Organism Pseudomonas aeruginosa strain PASGNDM345

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag B7W86_RS23600 Protein ID WP_003134109.1
Coordinates 4885117..4885527 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag B7W86_RS23605 Protein ID WP_170869361.1
Coordinates 4885527..4885769 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7W86_RS33395 4881399..4881812 - 414 WP_153570846.1 hypothetical protein -
B7W86_RS23560 4881823..4881963 + 141 WP_153549538.1 hypothetical protein -
B7W86_RS23565 4882120..4882848 + 729 WP_023094843.1 TIGR03761 family integrating conjugative element protein -
B7W86_RS23570 4882854..4883387 + 534 WP_003134105.1 DUF3158 family protein -
B7W86_RS23575 4883401..4883889 + 489 WP_003134107.1 single-stranded DNA-binding protein -
B7W86_RS23580 4883993..4884187 + 195 WP_031632157.1 hypothetical protein -
B7W86_RS23585 4884439..4884606 + 168 WP_128729268.1 CrpP family protein -
B7W86_RS23590 4884672..4884890 - 219 WP_023094844.1 hypothetical protein -
B7W86_RS23595 4884907..4885101 - 195 WP_023094845.1 hypothetical protein -
B7W86_RS23600 4885117..4885527 - 411 WP_003134109.1 tRNA(fMet)-specific endonuclease VapC Toxin
B7W86_RS23605 4885527..4885769 - 243 WP_170869361.1 antitoxin Antitoxin
B7W86_RS23610 4886075..4887994 + 1920 WP_003134111.1 type I DNA topoisomerase -
B7W86_RS23615 4888002..4888754 + 753 Protein_4559 hypothetical protein -
B7W86_RS23620 4888871..4889476 - 606 WP_023094848.1 LysE family translocator -
B7W86_RS23625 4889598..4890467 + 870 WP_023094849.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(11-37)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15452.78 Da        Isoelectric Point: 7.3233

>T75300 WP_003134109.1 NZ_CP020703:c4885527-4885117 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Download         Length: 411 bp

>T75300 NZ_CP020703:c4885527-4885117 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACCATCAAAAACCGTCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGTCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8909.94 Da        Isoelectric Point: 4.4230

>AT75300 WP_170869361.1 NZ_CP020703:c4885769-4885527 [Pseudomonas aeruginosa]
MRCVVEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Download         Length: 243 bp

>AT75300 NZ_CP020703:c4885769-4885527 [Pseudomonas aeruginosa]
ATGAGGTGTGTCGTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCT
TCCTGATGACGTTAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGTGAGTCTTGGGATA
GCTGGTTCGATGGCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References