Detailed information of TA system TA075295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 150295..150800
• Replicon: chromosome
• Accession: NZ_CP020703
• Organism: Pseudomonas aeruginosa strain PASGNDM345

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: B7W86_RS00695
• Protein ID: WP_003083773.1
• Coordinates: 150295..150576 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: B7W86_RS00700
• Protein ID: WP_003083775.1
• Coordinates: 150573..150800 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7W86_RS00670	145546..146895	+	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-
B7W86_RS00675	146944..147630	+	687	WP_003083762.1	FadR family transcriptional regulator	-
B7W86_RS00680	147731..148465	+	735	WP_003128333.1	GntR family transcriptional regulator	-
B7W86_RS00685	148669..149055	+	387	WP_003083767.1	aegerolysin family protein	-
B7W86_RS00690	149087..149995	-	909	WP_003083769.1	LysR family transcriptional regulator	-
B7W86_RS00695	150295..150576	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
B7W86_RS00700	150573..150800	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
B7W86_RS00705	150976..151596	-	621	WP_003101226.1	hypothetical protein	-
B7W86_RS00710	151697..152197	+	501	WP_003128336.1	LEA type 2 family protein	-
B7W86_RS00715	152270..152611	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
B7W86_RS00720	152693..154120	-	1428	WP_003083784.1	GABA permease	-
B7W86_RS00725	154289..155782	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T75295 WP_003083773.1 NZ_CP020703:c150576-150295 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T75295 NZ_CP020703:c150576-150295 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT75295 WP_003083775.1 NZ_CP020703:c150800-150573 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT75295 NZ_CP020703:c150800-150573 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9