Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3774958..3775592 Replicon chromosome
Accession NZ_CP020700
Organism Streptomyces tsukubensis strain NRRL 18488

Toxin (Protein)


Gene name vapC Uniprot ID I2N1L9
Locus tag B7R87_RS15165 Protein ID WP_006348208.1
Coordinates 3775170..3775592 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7G3UFR9
Locus tag B7R87_RS15160 Protein ID WP_040915655.1
Coordinates 3774958..3775173 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7R87_RS15140 3770596..3771807 - 1212 WP_040915676.1 radical SAM protein -
B7R87_RS15145 3771903..3772262 - 360 WP_006348212.1 hypothetical protein -
B7R87_RS15150 3772600..3773874 + 1275 WP_040915674.1 hypothetical protein -
B7R87_RS15155 3773855..3774514 - 660 WP_040915657.1 HAD family hydrolase -
B7R87_RS15160 3774958..3775173 + 216 WP_040915655.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
B7R87_RS15165 3775170..3775592 + 423 WP_006348208.1 PIN domain nuclease Toxin
B7R87_RS15170 3776049..3777353 + 1305 WP_006348207.1 cation:proton antiporter -
B7R87_RS15175 3777507..3777803 + 297 WP_078902262.1 type II toxin-antitoxin system RelE/ParE family toxin -
B7R87_RS15180 3777803..3778135 + 333 WP_006348205.1 helix-turn-helix transcriptional regulator -
B7R87_RS15185 3778296..3778628 + 333 WP_198965075.1 cupin domain-containing protein -
B7R87_RS15190 3778770..3779243 - 474 WP_006348203.1 carboxymuconolactone decarboxylase family protein -
B7R87_RS15195 3779333..3780151 - 819 WP_006348202.1 DUF817 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-46)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15468.37 Da        Isoelectric Point: 4.5876

>T75293 WP_006348208.1 NZ_CP020700:3775170-3775592 [Streptomyces tsukubensis]
VSAAQFLIDTSALARFLRGDAEQYGWDQAAAAGLIATCPITELEFFYSARSAEDRARGVEDLRMIFGWVPVDDRAYDRAW
QVQELLTRRGQHRSAGPVDLVVAATAELQGLTLLHRDRDFDCIAAVTGQALQWFGPDPGK

Download         Length: 423 bp

>T75293 NZ_CP020700:3775170-3775592 [Streptomyces tsukubensis]
GTGAGCGCGGCGCAGTTTCTGATCGACACCAGCGCGCTCGCCCGATTCCTCCGCGGGGACGCCGAGCAGTACGGCTGGGA
CCAGGCAGCAGCAGCCGGGCTCATCGCGACCTGCCCCATCACCGAGCTGGAGTTCTTCTACAGCGCGCGATCAGCCGAGG
ATCGCGCCCGGGGCGTCGAGGATCTGCGGATGATCTTCGGCTGGGTCCCCGTCGACGACCGCGCCTACGATCGCGCCTGG
CAGGTCCAGGAACTGCTGACTCGGCGCGGACAGCACCGCAGCGCCGGCCCGGTTGACCTCGTCGTCGCCGCCACAGCGGA
GTTGCAGGGCCTCACCCTCCTGCACCGCGACCGCGATTTCGACTGCATCGCCGCCGTCACCGGTCAGGCCCTGCAGTGGT
TCGGCCCCGATCCGGGCAAATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7893.87 Da        Isoelectric Point: 5.0634

>AT75293 WP_040915655.1 NZ_CP020700:3774958-3775173 [Streptomyces tsukubensis]
MSRTVIDLDDELLADVAQALGTSTKKETVNTALREVLENRRRALALARLRAAAADGGFDLDVFDDKQSYRR

Download         Length: 216 bp

>AT75293 NZ_CP020700:3774958-3775173 [Streptomyces tsukubensis]
ATGAGTCGCACAGTGATCGACCTGGACGACGAGCTGCTTGCCGACGTGGCGCAGGCGCTGGGAACCAGCACCAAGAAGGA
GACGGTCAATACCGCCCTGCGGGAAGTACTGGAGAACCGGCGCCGGGCTCTCGCTCTGGCCCGGCTGCGCGCGGCGGCGG
CCGACGGGGGCTTCGACCTGGACGTCTTCGACGACAAGCAGAGCTACCGGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2N1L9

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3UFR9

References