Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/DUF433(antitoxin)
Location 4843633..4844204 Replicon chromosome
Accession NZ_CP020664
Organism Microcystis sp. MC19

Toxin (Protein)


Gene name vapC Uniprot ID I4IBX6
Locus tag B5D77_RS24665 Protein ID WP_002759489.1
Coordinates 4843633..4843974 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1V4BQA4
Locus tag B5D77_RS24670 Protein ID WP_039899814.1
Coordinates 4843971..4844204 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5D77_RS24655 4839725..4840975 - 1251 WP_106909849.1 methionine adenosyltransferase -
B5D77_RS24660 4841302..4843623 + 2322 WP_106909850.1 DNA helicase PcrA -
B5D77_RS24665 4843633..4843974 - 342 WP_002759489.1 DUF5615 family PIN-like protein Toxin
B5D77_RS24670 4843971..4844204 - 234 WP_039899814.1 DUF433 domain-containing protein Antitoxin
B5D77_RS24675 4844530..4844958 + 429 WP_106909851.1 CAAD domain-containing protein -
B5D77_RS24680 4845164..4846516 - 1353 WP_008199989.1 glutathione-disulfide reductase -
B5D77_RS24685 4846691..4848115 + 1425 WP_008199991.1 D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Domain of unknown function DUF5615

(1-78)

Antitoxin

Protein of unknown function DUF433

(11-66)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12508.41 Da        Isoelectric Point: 4.2544

>T75289 WP_002759489.1 NZ_CP020664:c4843974-4843633 [Microcystis sp. MC19]
MKIWIDAQLPPTLASWLSATFDLEASSLRDLSLRDAQDIEIFAAARAENAVIMTKDSDFIDLVCRLGTPPQILWLTCGNV
TNQNLRRLLTATLPDALKQLEQGTIIVEISNTP

Download         Length: 342 bp

>T75289 NZ_CP020664:c4843974-4843633 [Microcystis sp. MC19]
ATGAAAATTTGGATTGATGCTCAACTACCTCCAACTCTAGCAAGCTGGTTATCAGCGACCTTTGATTTAGAAGCATCGTC
CCTGCGAGACTTATCCCTTCGAGATGCTCAAGATATCGAGATCTTTGCAGCAGCGCGAGCGGAAAATGCCGTGATTATGA
CCAAAGATAGTGACTTTATTGATTTGGTTTGTCGTCTGGGAACACCTCCTCAAATTTTATGGTTAACCTGTGGTAATGTC
ACAAATCAGAATCTGCGGCGATTACTGACAGCTACTTTACCCGATGCGTTGAAACAACTAGAGCAGGGAACAATAATTGT
AGAGATTAGCAATACTCCTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8499.91 Da        Isoelectric Point: 4.2677

>AT75289 WP_039899814.1 NZ_CP020664:c4844204-4843971 [Microcystis sp. MC19]
MDSTVSLLTRITQTPGQCGGRPCIRGMRIRVTDILEMLAENVSITEILEDFPDLELADIQACLLFAARCTDFPKLTA

Download         Length: 234 bp

>AT75289 NZ_CP020664:c4844204-4843971 [Microcystis sp. MC19]
ATGGACTCAACAGTTAGCTTGCTTACCCGCATTACTCAAACACCCGGCCAGTGTGGTGGTCGTCCTTGTATTCGGGGGAT
GCGAATTCGAGTAACGGATATTTTGGAAATGTTAGCGGAGAATGTGAGCATAACTGAAATTCTAGAAGACTTTCCCGATT
TGGAACTCGCAGATATCCAAGCTTGTTTACTTTTTGCAGCACGGTGTACGGATTTTCCTAAACTAACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I4IBX6

Antitoxin

Source ID Structure
AlphaFold DB A0A1V4BQA4

References