Detailed information of TA system TA075282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 966366..966784
• Replicon: chromosome
• Accession: NZ_CP020664
• Organism: Microcystis sp. MC19

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: B5D77_RS04925
• Protein ID: WP_106908389.1
• Coordinates: 966366..966578 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: I4FWC9
• Locus tag: B5D77_RS04930
• Protein ID: WP_002743279.1
• Coordinates: 966578..966784 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5D77_RS04910	962159..963181	+	1023	WP_106908386.1	DNA (cytosine-5-)-methyltransferase	-
B5D77_RS04915	963185..964255	+	1071	WP_106908387.1	HaeII family restriction endonuclease	-
B5D77_RS04920	964847..966331	+	1485	WP_106908388.1	GUN4 domain-containing protein	-
B5D77_RS04925	966366..966578	+	213	WP_106908389.1	type II toxin-antitoxin system HicA family toxin	Toxin
B5D77_RS04930	966578..966784	+	207	WP_002743279.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B5D77_RS04940	966946..967146	+	201	WP_061433428.1	hypothetical protein	-
B5D77_RS04945	967140..967334	+	195	WP_002789683.1	hypothetical protein	-
B5D77_RS04950	967384..967704	+	321	WP_002734685.1	hypothetical protein	-
B5D77_RS04955	967717..967953	+	237	WP_002792164.1	DUF4926 domain-containing protein	-
B5D77_RS04960	968465..968893	+	429	WP_008198370.1	hypothetical protein	-
B5D77_RS04965	968890..969102	+	213	WP_002763392.1	hypothetical protein	-
B5D77_RS04970	969234..969467	+	234	WP_002775448.1	DUF433 domain-containing protein	-
B5D77_RS04975	969660..969911	+	252	WP_106908391.1	hypothetical protein	-
B5D77_RS04980	969904..970203	+	300	WP_106908392.1	hypothetical protein	-
B5D77_RS04985	970234..970446	+	213	WP_008198381.1	hypothetical protein	-
B5D77_RS04990	970433..970705	+	273	WP_106908393.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-63)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8072.20 Da
• Isoelectric Point: 10.3151

>T75282 WP_106908389.1 NZ_CP020664:966366-966578 [Microcystis sp. MC19]
MKVRDVIRLLEQDGWYLSRTRGSHRQFKHSLKSGLVTVPGKPSDDLAIKTLQSIFKQSQLNKYLEEGDNN

Nucleotide

• Length: 213 bp

>T75282 NZ_CP020664:966366-966578 [Microcystis sp. MC19]
ATGAAAGTTAGAGATGTAATTAGATTACTAGAACAAGATGGATGGTATTTATCGAGAACAAGGGGAAGCCACCGACAATT
CAAACATTCTCTGAAATCAGGTTTAGTTACTGTTCCGGGTAAGCCATCCGATGATTTAGCGATTAAGACTTTACAGAGTA
TTTTCAAACAATCTCAATTGAATAAATATCTAGAAGAAGGAGACAATAACTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7663.68 Da
• Isoelectric Point: 4.0270

>AT75282 WP_002743279.1 NZ_CP020664:966578-966784 [Microcystis sp. MC19]
MRYAIIIEKAENNYSAYVPDLPGCVTTGKTLEEITENMKEAIQFHLDGLQEEGLPIPQPATYCQYIEA

Nucleotide

• Length: 207 bp

>AT75282 NZ_CP020664:966578-966784 [Microcystis sp. MC19]
ATGCGTTACGCGATTATTATCGAAAAAGCTGAAAATAACTATTCCGCTTATGTTCCCGATTTACCCGGTTGTGTTACTAC
AGGTAAAACCCTAGAGGAAATCACAGAAAATATGAAAGAAGCAATTCAATTTCATCTGGATGGATTACAAGAGGAAGGGC
TGCCAATTCCCCAACCAGCCACCTATTGCCAGTATATCGAAGCGTAA

Structures

Antitoxin

• AlphaFold DB: I4FWC9