Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 545363..545976 Replicon chromosome
Accession NZ_CP020664
Organism Microcystis sp. MC19

Toxin (Protein)


Gene name vapC Uniprot ID A0A2P1UBV3
Locus tag B5D77_RS02850 Protein ID WP_039899913.1
Coordinates 545590..545976 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID I4ICX3
Locus tag B5D77_RS02845 Protein ID WP_008200865.1
Coordinates 545363..545590 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5D77_RS02830 542464..543651 + 1188 WP_106908238.1 AAA family ATPase -
B5D77_RS02835 543676..544287 + 612 WP_039899916.1 DUF4276 family protein -
B5D77_RS02840 544842..545321 + 480 WP_008200866.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
B5D77_RS02845 545363..545590 + 228 WP_008200865.1 DUF2281 domain-containing protein Antitoxin
B5D77_RS02850 545590..545976 + 387 WP_039899913.1 type II toxin-antitoxin system VapC family toxin Toxin
B5D77_RS02855 546069..547826 + 1758 WP_106908239.1 arginine--tRNA ligase -
B5D77_RS02860 548560..548952 + 393 WP_008200859.1 Mo-dependent nitrogenase C-terminal domain-containing protein -
B5D77_RS02865 548983..549741 + 759 WP_008200857.1 hypothetical protein -
B5D77_RS02870 549746..549997 + 252 WP_008200855.1 hypothetical protein -
B5D77_RS02875 550040..550447 - 408 WP_008200854.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-127)

Antitoxin

Domain of unknown function DUF2281

(6-74)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14844.07 Da        Isoelectric Point: 4.8942

>T75281 WP_039899913.1 NZ_CP020664:545590-545976 [Microcystis sp. MC19]
MTSFLLDTHTFIWLTENDSNLPNNLREEIDFAPEVYVSIVSLWEIAIKLNLGKLDLQKSYETIETKLEASDITLLSISFS
DTLKICTLPLHHRDPFDRMLIAQSLNRSLILISKDTKFDAYNVPRKWT

Download         Length: 387 bp

>T75281 NZ_CP020664:545590-545976 [Microcystis sp. MC19]
ATGACATCTTTTCTTCTGGATACTCATACTTTCATCTGGTTAACTGAAAACGATTCTAACTTGCCTAATAATCTTCGGGA
GGAAATAGATTTTGCTCCTGAAGTTTATGTCAGTATTGTTAGTCTCTGGGAAATTGCGATTAAGTTAAATTTAGGTAAGT
TAGACCTACAAAAAAGTTATGAAACCATTGAAACTAAACTAGAAGCATCAGATATAACCTTACTGTCTATATCATTTTCT
GATACTCTAAAAATTTGTACTTTACCCTTGCATCACCGTGATCCATTTGATAGAATGCTAATTGCTCAGTCTCTAAACAG
ATCACTAATTCTAATTAGTAAAGATACTAAATTTGATGCTTATAATGTACCAAGAAAATGGACATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8785.02 Da        Isoelectric Point: 4.1064

>AT75281 WP_008200865.1 NZ_CP020664:545363-545590 [Microcystis sp. MC19]
MNIDTKILETVEAMPELLKIELLHYAEYLMANYQSDVIIEKPSPRKRRSGILQGTFTLPLSDDFDESLEDFQEYM

Download         Length: 228 bp

>AT75281 NZ_CP020664:545363-545590 [Microcystis sp. MC19]
ATGAATATAGACACAAAAATCTTAGAAACTGTCGAAGCAATGCCAGAGTTATTAAAAATAGAACTGCTTCACTATGCTGA
ATATTTGATGGCCAATTATCAATCAGATGTTATTATAGAGAAACCTTCTCCTAGAAAACGTCGTTCAGGAATTTTACAAG
GTACATTTACCTTACCTTTGTCTGATGATTTTGATGAGTCTTTGGAAGATTTTCAGGAATATATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1UBV3

Antitoxin

Source ID Structure
AlphaFold DB I4ICX3

References