Detailed information of TA system TA075279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 419111..419751
• Replicon: chromosome
• Accession: NZ_CP020664
• Organism: Microcystis sp. MC19

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B5D77_RS02160
• Protein ID: WP_106908191.1
• Coordinates: 419111..419509 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I4ID84
• Locus tag: B5D77_RS02165
• Protein ID: WP_008201147.1
• Coordinates: 419506..419751 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5D77_RS02120	414337..414600	+	264	WP_002799510.1	DUF433 domain-containing protein	-
B5D77_RS02125	414584..414910	+	327	WP_084990056.1	DUF5615 family PIN-like protein	-
B5D77_RS02130	414937..415557	-	621	Protein_398	agmatinase	-
B5D77_RS02135	416068..416418	-	351	WP_106908187.1	DUF86 domain-containing protein	-
B5D77_RS02140	416408..416698	-	291	WP_002757283.1	nucleotidyltransferase	-
B5D77_RS02145	416962..417462	-	501	WP_106908188.1	ATPase	-
B5D77_RS02150	417620..418357	+	738	WP_106908189.1	PEP-CTERM sorting domain-containing protein	-
B5D77_RS02155	418526..418789	+	264	WP_106908190.1	DUF2442 domain-containing protein	-
B5D77_RS02160	419111..419509	-	399	WP_106908191.1	type II toxin-antitoxin system VapC family toxin	Toxin
B5D77_RS02165	419506..419751	-	246	WP_008201147.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B5D77_RS02170	420089..420364	+	276	WP_002739245.1	hypothetical protein	-
B5D77_RS02175	420361..420726	+	366	WP_106908192.1	clan AA aspartic protease	-
B5D77_RS02180	421026..421826	+	801	WP_008201141.1	uroporphyrinogen-III C-methyltransferase	-
B5D77_RS02185	421837..422256	+	420	WP_008201139.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
B5D77_RS02190	422389..422883	+	495	WP_002748906.1	photosystem I reaction center subunit III	-
B5D77_RS02195	422929..423057	+	129	WP_002738148.1	photosystem I reaction center subunit IX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-41)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14973.02 Da
• Isoelectric Point: 4.4046

>T75279 WP_106908191.1 NZ_CP020664:c419509-419111 [Microcystis sp. MC19]
VRLLLDTQCWLWWFAQPDKLSENVIEQIANESNELWFSVASVWEMGIKVSVGKLPLPEQIDDYVSTRMTQLGARSLEINA
SHALRAAALPLHHRDPFDRMLIAQAQVEDMTLVSADSTFNQYEVSLLWAASS

Nucleotide

• Length: 399 bp

>T75279 NZ_CP020664:c419509-419111 [Microcystis sp. MC19]
GTGAGACTTTTACTGGATACACAATGCTGGCTGTGGTGGTTTGCCCAACCAGACAAGTTAAGTGAGAATGTAATTGAACA
AATTGCAAACGAAAGCAATGAATTGTGGTTTTCTGTTGCCAGTGTTTGGGAGATGGGAATTAAAGTCTCAGTCGGTAAAT
TACCACTGCCAGAGCAAATAGATGATTATGTCTCTACTCGAATGACTCAACTAGGAGCTAGATCGTTAGAGATCAATGCT
TCTCATGCTTTGCGGGCGGCTGCATTGCCTTTACATCATCGTGATCCTTTTGACCGAATGCTGATTGCACAAGCTCAGGT
AGAAGATATGACACTTGTGAGTGCGGATTCAACATTTAATCAATATGAAGTGTCTTTGCTTTGGGCAGCTAGTTCTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8950.20 Da
• Isoelectric Point: 4.7361

>AT75279 WP_008201147.1 NZ_CP020664:c419751-419506 [Microcystis sp. MC19]
METVNIHQAKTNLSRLLSRVELGEEIVISNRGIPIAKLVPFRTSLDRRSSLGQDRGMFTVPDDFNAPLPEDILVAFEGGA
E

Nucleotide

• Length: 246 bp

>AT75279 NZ_CP020664:c419751-419506 [Microcystis sp. MC19]
ATGGAAACTGTAAACATTCATCAGGCTAAAACAAACCTTTCTCGGCTATTGTCCCGTGTGGAACTCGGAGAGGAAATCGT
TATTTCAAACCGAGGTATTCCAATTGCTAAACTTGTTCCATTTCGGACATCTTTAGATCGACGGTCTAGTTTAGGACAAG
ATCGAGGGATGTTTACCGTACCAGATGATTTTAACGCTCCTTTACCAGAAGATATTTTGGTAGCATTTGAGGGTGGTGCG
GAGTGA

Structures

Antitoxin

• AlphaFold DB: I4ID84