Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4973320..4973977 Replicon chromosome
Accession NZ_CP020657
Organism Klebsiella sp. M5al

Toxin (Protein)


Gene name cptA Uniprot ID A0A285B9I7
Locus tag BWI76_RS23825 Protein ID WP_004134421.1
Coordinates 4973320..4973730 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A285B945
Locus tag BWI76_RS23830 Protein ID WP_004105561.1
Coordinates 4973711..4973977 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI76_RS23805 4969297..4971030 - 1734 WP_004134426.1 single-stranded-DNA-specific exonuclease RecJ -
BWI76_RS23810 4971036..4971749 - 714 WP_004134425.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BWI76_RS23815 4971772..4972668 - 897 WP_004124942.1 site-specific tyrosine recombinase XerD -
BWI76_RS23820 4972790..4973311 + 522 WP_004134423.1 flavodoxin FldB -
BWI76_RS23825 4973320..4973730 - 411 WP_004134421.1 protein YgfX Toxin
BWI76_RS23830 4973711..4973977 - 267 WP_004105561.1 FAD assembly factor SdhE Antitoxin
BWI76_RS23835 4974221..4975204 + 984 WP_049087337.1 tRNA-modifying protein YgfZ -
BWI76_RS23840 4975331..4975990 - 660 WP_004124978.1 hemolysin III family protein -
BWI76_RS23845 4976153..4976464 - 312 WP_004133599.1 N(4)-acetylcytidine aminohydrolase -
BWI76_RS23850 4976515..4977243 + 729 WP_024358621.1 MurR/RpiR family transcriptional regulator -
BWI76_RS23855 4977365..4978798 + 1434 WP_004133593.1 6-phospho-beta-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16075.86 Da        Isoelectric Point: 10.9455

>T75273 WP_004134421.1 NZ_CP020657:c4973730-4973320 [Klebsiella sp. M5al]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLVLPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIVGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLQKPTQE

Download         Length: 411 bp

>T75273 NZ_CP020657:c4973730-4973320 [Klebsiella sp. M5al]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCAGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGTCTGCGGAATACGGAAAACCAACGTAC
CCAGCATTTATGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGCGACCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10555.02 Da        Isoelectric Point: 5.1498

>AT75273 WP_004105561.1 NZ_CP020657:c4973977-4973711 [Klebsiella sp. M5al]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDDDKRLFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT75273 NZ_CP020657:c4973977-4973711 [Klebsiella sp. M5al]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285B9I7

Antitoxin

Source ID Structure
AlphaFold DB A0A285B945

References