Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4535636..4536419 Replicon chromosome
Accession NZ_CP020657
Organism Klebsiella sp. M5al

Toxin (Protein)


Gene name yeeV Uniprot ID A0A4R0G0V7
Locus tag BWI76_RS21685 Protein ID WP_004136885.1
Coordinates 4536045..4536419 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A4R0FW90
Locus tag BWI76_RS21680 Protein ID WP_004136886.1
Coordinates 4535636..4535995 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI76_RS21635 4530864..4531721 + 858 WP_004136905.1 hypothetical protein -
BWI76_RS21640 4531999..4532463 + 465 WP_004136903.1 hypothetical protein -
BWI76_RS21645 4532518..4532877 + 360 WP_004136901.1 hypothetical protein -
BWI76_RS21650 4532963..4533175 + 213 WP_004136899.1 hypothetical protein -
BWI76_RS21655 4533282..4534103 + 822 WP_004136895.1 DUF945 domain-containing protein -
BWI76_RS21660 4534182..4534655 + 474 WP_004136892.1 antirestriction protein -
BWI76_RS21670 4534896..4535378 + 483 WP_004136890.1 DNA repair protein RadC -
BWI76_RS21675 4535392..4535613 + 222 WP_064371656.1 DUF987 domain-containing protein -
BWI76_RS21680 4535636..4535995 + 360 WP_004136886.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BWI76_RS21685 4536045..4536419 + 375 WP_004136885.1 TA system toxin CbtA family protein Toxin
BWI76_RS21690 4536499..4536819 - 321 WP_004136883.1 hypothetical protein -
BWI76_RS21695 4536958..4537947 - 990 WP_004136877.1 glycosyltransferase -
BWI76_RS21700 4538613..4539407 - 795 WP_012540031.1 helix-turn-helix transcriptional regulator -
BWI76_RS21705 4539495..4540685 + 1191 WP_004136868.1 CynX/NimT family MFS transporter -
BWI76_RS21710 4540857..4541231 - 375 Protein_4167 nitroreductase family protein -
BWI76_RS21715 4541222..4541401 + 180 Protein_4168 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4517330..4570943 53613


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(8-112)

Antitoxin

Antitoxin CbeA

(17-114)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13889.84 Da        Isoelectric Point: 9.8173

>T75272 WP_004136885.1 NZ_CP020657:4536045-4536419 [Klebsiella sp. M5al]
MQIQSLSPQRAASSRLSSVESWRKLLEYLLKQHYGLMMNDTPFGDDSVIQKNIDAGISLCDAVNFIVEKYGLVRTDRHGF
SVTEQSPFIDSIDMLRARKATGLMTRKGYQTVTDITSGRSRGGK

Download         Length: 375 bp

>T75272 NZ_CP020657:4536045-4536419 [Klebsiella sp. M5al]
ATGCAAATACAATCCCTGTCCCCTCAAAGGGCGGCCTCGTCACGTCTGTCGTCAGTCGAAAGCTGGCGAAAATTGCTGGA
GTATCTGCTGAAACAACACTACGGTCTGATGATGAACGACACACCGTTTGGCGACGACAGTGTGATCCAGAAGAACATTG
ATGCGGGAATCTCACTGTGTGATGCGGTGAATTTTATCGTGGAAAAGTACGGTCTGGTGCGTACTGACCGTCACGGCTTC
AGTGTGACAGAGCAGTCTCCGTTCATCGACAGCATCGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGCTATCAAACCGTTACTGACATCACCAGCGGCAGATCCAGAGGAGGGAAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13300.44 Da        Isoelectric Point: 7.7515

>AT75272 WP_004136886.1 NZ_CP020657:4535636-4535995 [Klebsiella sp. M5al]
MQKATRVINHNIAEPWRGLRRNITPCFGARLVQEGNHLHYLADRASIAGTFNEADLRYLDQAFPVLMKQMELMLASSELT
PHIQRCITLHAKGLICEADTLGSCGYLFIVIYPTSAISA

Download         Length: 360 bp

>AT75272 NZ_CP020657:4535636-4535995 [Klebsiella sp. M5al]
ATGCAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGCGGGGCCTCAGACGCAACATCACACCGTGTTT
TGGTGCACGGCTGGTACAGGAGGGTAACCACCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGAAG
CAGATTTACGCTATCTGGACCAGGCATTTCCGGTACTGATGAAGCAAATGGAGCTTATGCTGGCCAGCAGCGAACTCACT
CCCCACATTCAGCGCTGCATCACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACCCTCGGCTCCTGTGGCTATTT
GTTCATCGTAATTTATCCGACATCTGCAATAAGCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G0V7

Antitoxin

Source ID Structure
AlphaFold DB A0A4R0FW90

References