Detailed information of TA system TA075262

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2149726..2150509
• Replicon: chromosome
• Accession: NZ_CP020657
• Organism: Klebsiella sp. M5al

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BWI76_RS10130
• Protein ID: WP_164852945.1
• Coordinates: 2149726..2150094 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BWI76_RS10135
• Protein ID: WP_049094917.1
• Coordinates: 2150150..2150509 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI76_RS10105	2145406..2145639	-	234	WP_004132711.1	hypothetical protein	-
BWI76_RS28305	2146049..2146321	+	273	WP_192831626.1	hypothetical protein	-
BWI76_RS10115	2146524..2147432	+	909	WP_049094914.1	kdo(2)-lipid IV(A) palmitoleoyltransferase	-
BWI76_RS10120	2148198..2149187	+	990	WP_049094915.1	glycosyltransferase	-
BWI76_RS10125	2149326..2149646	+	321	WP_049094934.1	hypothetical protein	-
BWI76_RS10130	2149726..2150094	-	369	WP_164852945.1	TA system toxin CbtA family protein	Toxin
BWI76_RS10135	2150150..2150509	-	360	WP_049094917.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BWI76_RS10140	2150532..2150753	-	222	WP_049094918.1	DUF987 domain-containing protein	-
BWI76_RS10145	2150767..2151249	-	483	WP_049094919.1	DNA repair protein RadC	-
BWI76_RS10150	2151246..2151479	-	234	WP_049094920.1	hypothetical protein	-
BWI76_RS10155	2151490..2151927	-	438	WP_049094935.1	antirestriction protein	-
BWI76_RS10160	2152049..2152870	-	822	WP_049094921.1	DUF945 domain-containing protein	-
BWI76_RS10165	2152977..2153189	-	213	WP_049094922.1	hypothetical protein	-
BWI76_RS10170	2153299..2153772	-	474	WP_049094923.1	hypothetical protein	-
BWI76_RS10175	2153846..2154475	-	630	WP_049094924.1	hypothetical protein	-
BWI76_RS10180	2154573..2155250	-	678	WP_004139645.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2146049..2169282	23233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (7-110)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13672.62 Da
• Isoelectric Point: 9.0625

>T75262 WP_164852945.1 NZ_CP020657:c2150094-2149726 [Klebsiella sp. M5al]
IQSLPPQRAASSRLSSVEIWQKLLEYLLEQHYGLTLNNTPFCNDSVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGFSV
KEQSPFIGSIDMLRARKATGLMPRKGYQTVTDITSGRFSGGK

Nucleotide

• Length: 369 bp

>T75262 NZ_CP020657:c2150094-2149726 [Klebsiella sp. M5al]
ATACAATCCCTGCCCCCACAAAGGGCGGCATCGTCACGTCTGTCATCAGTCGAAATCTGGCAAAAATTACTGGAGTATCT
GCTGGAGCAACACTACGGCCTTACCCTCAACAACACACCGTTTTGCAACGACAGTGTGATCCAGAAGCACATTGATGCGG
GTATCTCACTGTGTGACGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTCAGCGTG
AAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCCGCAAGGCTACCGGGCTGATGCCCCGCAAGGGCTA
TCAAACCGTTACTGACATTACCAGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13358.40 Da
• Isoelectric Point: 7.3727

>AT75262 WP_049094917.1 NZ_CP020657:c2150509-2150150 [Klebsiella sp. M5al]
MQKATRVINHNIAEPWWGLRRNITPCFGARLVQEGNHLHYLADRASIAGTFSDADLRHLDQAFPVVMKQMELMLTSNELT
PHIQRCITFHAKGLICEADTLGSCGYLYIVIYPTSATTA

Nucleotide

• Length: 360 bp

>AT75262 NZ_CP020657:c2150509-2150150 [Klebsiella sp. M5al]
ATGCAGAAAGCAACCCGTGTAATCAACCATAATATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
TGGTGCCCGGCTTGTACAGGAGGGTAACCACCTGCATTACCTAGCTGACCGCGCCAGCATTGCTGGGACATTCAGCGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTAGTGATGAAGCAGATGGAGCTCATGCTGACCAGCAACGAACTCACG
CCCCACATTCAGCGCTGCATCACGTTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATCT
TTACATCGTAATTTATCCGACATCTGCAACAACCGCATAA