Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1417755..1418374 Replicon chromosome
Accession NZ_CP020657
Organism Klebsiella sp. M5al

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag BWI76_RS06765 Protein ID WP_004099646.1
Coordinates 1417755..1417973 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag BWI76_RS06770 Protein ID WP_004129911.1
Coordinates 1418000..1418374 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI76_RS06735 1413802..1414065 + 264 WP_004129897.1 type B 50S ribosomal protein L31 -
BWI76_RS06740 1414065..1414205 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
BWI76_RS06745 1414202..1414900 - 699 WP_004136044.1 GNAT family N-acetyltransferase -
BWI76_RS06750 1415001..1416455 + 1455 WP_004136046.1 PLP-dependent aminotransferase family protein -
BWI76_RS06755 1416430..1416900 - 471 WP_004136048.1 YlaC family protein -
BWI76_RS06760 1417027..1417593 - 567 WP_004136050.1 maltose O-acetyltransferase -
BWI76_RS06765 1417755..1417973 - 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
BWI76_RS06770 1418000..1418374 - 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
BWI76_RS06775 1418843..1421989 - 3147 WP_004848339.1 multidrug efflux RND transporter permease subunit -
BWI76_RS06780 1422012..1423205 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T75261 WP_004099646.1 NZ_CP020657:c1417973-1417755 [Klebsiella sp. M5al]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T75261 NZ_CP020657:c1417973-1417755 [Klebsiella sp. M5al]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCCGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT75261 WP_004129911.1 NZ_CP020657:c1418374-1418000 [Klebsiella sp. M5al]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT75261 NZ_CP020657:c1418374-1418000 [Klebsiella sp. M5al]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATTACTCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGTGCGGATCTGCAGAAATGGCGCAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References