Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 91293..91903 Replicon chromosome
Accession NZ_CP020657
Organism Klebsiella sp. M5al

Toxin (Protein)


Gene name vapC Uniprot ID A0A9E1GAR7
Locus tag BWI76_RS00445 Protein ID WP_049086919.1
Coordinates 91529..91903 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag BWI76_RS00440 Protein ID WP_077265771.1
Coordinates 91293..91532 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI76_RS00415 87279..87878 + 600 WP_004132872.1 glucose-1-phosphatase -
BWI76_RS00420 87872..88732 + 861 WP_004127107.1 virulence factor BrkB family protein -
BWI76_RS00425 88729..89166 + 438 WP_004132870.1 D-tyrosyl-tRNA(Tyr) deacylase -
BWI76_RS00430 89211..90152 + 942 WP_004127112.1 fatty acid biosynthesis protein FabY -
BWI76_RS00435 90170..91087 - 918 WP_024360706.1 alpha/beta hydrolase -
BWI76_RS00440 91293..91532 + 240 WP_077265771.1 ribbon-helix-helix domain-containing protein Antitoxin
BWI76_RS00445 91529..91903 + 375 WP_049086919.1 type II toxin-antitoxin system VapC family toxin Toxin
BWI76_RS00450 91941..92870 - 930 WP_004132861.1 formate dehydrogenase accessory protein FdhE -
BWI76_RS00455 93214..93849 - 636 WP_004132860.1 formate dehydrogenase cytochrome b556 subunit -
BWI76_RS00460 93846..94748 - 903 WP_004122548.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14111.45 Da        Isoelectric Point: 8.4571

>T75259 WP_049086919.1 NZ_CP020657:91529-91903 [Klebsiella sp. M5al]
MIKGPALFDTNILIDLFSGRVEAKQVLEAYPPQNAISLITWMEVMVGAKKYHQEHRTRIALSAFNIIDISQDIAERSVNL
RKEYGMKLPDAIILATAQIHRYELVTRNTKDFFGIPGVITPYHL

Download         Length: 375 bp

>T75259 NZ_CP020657:91529-91903 [Klebsiella sp. M5al]
ATGATTAAAGGCCCGGCGCTGTTTGATACTAATATTCTCATCGATTTATTCAGCGGTCGCGTTGAAGCGAAACAGGTTCT
GGAGGCTTATCCGCCACAGAATGCGATTAGCCTCATCACGTGGATGGAAGTAATGGTGGGGGCGAAAAAGTATCATCAGG
AACACCGCACGCGTATTGCACTGAGCGCGTTTAATATTATCGATATTTCACAGGATATTGCTGAACGAAGCGTCAATCTC
AGGAAGGAGTATGGGATGAAGCTGCCGGATGCCATCATACTGGCGACAGCGCAAATACATCGTTATGAACTGGTCACGCG
CAATACCAAAGATTTTTTCGGGATACCCGGAGTGATTACGCCTTATCATTTGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9307.51 Da        Isoelectric Point: 4.3253

>AT75259 WP_077265771.1 NZ_CP020657:91293-91532 [Klebsiella sp. M5al]
MMTGTNMGRILLDLSDDVIKRLEDLKQQRNLPRAELLREAVEQYLERQADTTISNALGIWQGCEEEGVEFERKLREEWQ

Download         Length: 240 bp

>AT75259 NZ_CP020657:91293-91532 [Klebsiella sp. M5al]
ATGATGACGGGAACGAATATGGGCAGAATACTACTCGACTTATCGGATGACGTTATTAAACGTCTTGAAGACCTCAAACA
GCAGCGCAATCTTCCTCGAGCGGAACTGTTACGCGAGGCGGTTGAACAGTACCTGGAAAGACAAGCTGACACCACTATTT
CCAATGCGCTGGGGATTTGGCAAGGCTGTGAGGAAGAGGGAGTTGAATTCGAAAGGAAACTCCGCGAGGAGTGGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References