Detailed information of TA system TA075230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3195812..3196343
• Replicon: chromosome
• Accession: NZ_CP020651
• Organism: Bordetella bronchiseptica strain D755

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: B7P00_RS15010
• Protein ID: WP_003812626.1
• Coordinates: 3195812..3196078 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: B7P00_RS15015
• Protein ID: WP_003812628.1
• Coordinates: 3196065..3196343 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7P00_RS14985	3190939..3191211	+	273	WP_003812615.1	hypothetical protein	-
B7P00_RS14990	3191250..3191960	-	711	WP_003812617.1	aquaporin Z	-
B7P00_RS14995	3192071..3192703	-	633	WP_003812619.1	PAS domain-containing protein	-
B7P00_RS15000	3193100..3193978	+	879	WP_003812621.1	tripartite tricarboxylate transporter substrate binding protein	-
B7P00_RS15005	3194040..3195581	+	1542	WP_003812624.1	M81 family metallopeptidase	-
B7P00_RS15010	3195812..3196078	+	267	WP_003812626.1	BrnT family toxin	Toxin
B7P00_RS15015	3196065..3196343	+	279	WP_003812628.1	BrnA antitoxin family protein	Antitoxin
B7P00_RS15025	3196637..3197293	-	657	WP_003812630.1	phosphoribosylanthranilate isomerase	-
B7P00_RS15030	3197308..3198975	-	1668	WP_003812634.1	TRAP transporter large permease subunit	-
B7P00_RS15035	3198978..3199607	-	630	WP_033451894.1	TRAP transporter small permease subunit	-
B7P00_RS15040	3199819..3200913	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T75230 WP_003812626.1 NZ_CP020651:3195812-3196078 [Bordetella bronchiseptica]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T75230 NZ_CP020651:3195812-3196078 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10426.92 Da
• Isoelectric Point: 10.5548

>AT75230 WP_003812628.1 NZ_CP020651:3196065-3196343 [Bordetella bronchiseptica]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQIVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT75230 NZ_CP020651:3196065-3196343 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGA
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Toxin

• AlphaFold DB: K0MH61