Detailed information of TA system TA075226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3200114..3200645
• Replicon: chromosome
• Accession: NZ_CP020650
• Organism: Bordetella bronchiseptica strain E001

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: B7P04_RS15050
• Protein ID: WP_003812626.1
• Coordinates: 3200114..3200380 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: B7P04_RS15055
• Protein ID: WP_003816743.1
• Coordinates: 3200367..3200645 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7P04_RS15025	3195241..3195513	+	273	WP_003812615.1	hypothetical protein	-
B7P04_RS15030	3195552..3196262	-	711	WP_003812617.1	aquaporin Z	-
B7P04_RS15035	3196373..3197005	-	633	WP_003812619.1	PAS domain-containing protein	-
B7P04_RS15040	3197402..3198280	+	879	WP_033450228.1	tripartite tricarboxylate transporter substrate binding protein	-
B7P04_RS15045	3198342..3199883	+	1542	WP_010928296.1	M81 family metallopeptidase	-
B7P04_RS15050	3200114..3200380	+	267	WP_003812626.1	BrnT family toxin	Toxin
B7P04_RS15055	3200367..3200645	+	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
B7P04_RS15065	3200953..3201609	-	657	WP_033456960.1	phosphoribosylanthranilate isomerase	-
B7P04_RS15070	3201624..3203291	-	1668	WP_003812634.1	TRAP transporter large permease subunit	-
B7P04_RS15075	3203294..3203923	-	630	WP_003812635.1	TRAP transporter small permease subunit	-
B7P04_RS15080	3204135..3205229	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T75226 WP_003812626.1 NZ_CP020650:3200114-3200380 [Bordetella bronchiseptica]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T75226 NZ_CP020650:3200114-3200380 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT75226 WP_003816743.1 NZ_CP020650:3200367-3200645 [Bordetella bronchiseptica]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT75226 NZ_CP020650:3200367-3200645 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Toxin

• AlphaFold DB: K0MH61

Antitoxin

• AlphaFold DB: K0MF02