Detailed information of TA system TA075208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3224477..3225008
• Replicon: chromosome
• Accession: NZ_CP020645
• Organism: Bordetella bronchiseptica strain I943

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: B7P02_RS15205
• Protein ID: WP_003816744.1
• Coordinates: 3224477..3224743 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: B7P02_RS15210
• Protein ID: WP_003816743.1
• Coordinates: 3224730..3225008 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7P02_RS15180	3219786..3220496	-	711	WP_003816747.1	aquaporin Z	-
B7P02_RS15185	3220604..3221236	-	633	WP_003812619.1	PAS domain-containing protein	-
B7P02_RS15190	3221633..3222511	+	879	WP_033453539.1	tripartite tricarboxylate transporter substrate binding protein	-
B7P02_RS15195	3222573..3224115	+	1543	Protein_2990	M81 family metallopeptidase	-
B7P02_RS15200	3224169..3224359	+	191	Protein_2991	IS481 family transposase	-
B7P02_RS15205	3224477..3224743	+	267	WP_003816744.1	BrnT family toxin	Toxin
B7P02_RS15210	3224730..3225008	+	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
B7P02_RS24720	3225059..3225199	+	141	WP_157966511.1	hypothetical protein	-
B7P02_RS15220	3225350..3226006	-	657	WP_015041647.1	phosphoribosylanthranilate isomerase	-
B7P02_RS15225	3226021..3227688	-	1668	WP_015041648.1	TRAP transporter large permease subunit	-
B7P02_RS15230	3227691..3228320	-	630	WP_010930386.1	TRAP transporter small permease subunit	-
B7P02_RS15235	3228532..3229626	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10032.44 Da
• Isoelectric Point: 9.4798

>T75208 WP_003816744.1 NZ_CP020645:3224477-3224743 [Bordetella bronchiseptica]
MDITYDPAKNDKNIADRGLSFELVRGFEWASALVVEDARQAYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T75208 NZ_CP020645:3224477-3224743 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGCGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATGCCCGCCAGGCCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT75208 WP_003816743.1 NZ_CP020645:3224730-3225008 [Bordetella bronchiseptica]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT75208 NZ_CP020645:3224730-3225008 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCAATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Antitoxin

• AlphaFold DB: K0MF02