Detailed information of TA system TA075178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 147775..148280
• Replicon: chromosome
• Accession: NZ_CP020603
• Organism: Pseudomonas aeruginosa strain E6130952

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: E613_RS00865
• Protein ID: WP_003083773.1
• Coordinates: 147775..148056 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: E613_RS00870
• Protein ID: WP_003083775.1
• Coordinates: 148053..148280 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E613_RS00840	143026..144375	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
E613_RS00845	144424..145110	+	687	WP_003083762.1	FadR family transcriptional regulator	-
E613_RS00850	145211..145945	+	735	WP_003083764.1	GntR family transcriptional regulator	-
E613_RS00855	146149..146535	+	387	WP_003083767.1	aegerolysin family protein	-
E613_RS00860	146567..147475	-	909	WP_003083769.1	LysR family transcriptional regulator	-
E613_RS00865	147775..148056	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
E613_RS00870	148053..148280	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
E613_RS00875	148456..149076	-	621	WP_003101226.1	hypothetical protein	-
E613_RS00880	149177..149677	+	501	WP_003083778.1	LEA type 2 family protein	-
E613_RS00885	149750..150091	+	342	WP_014603467.1	alkylphosphonate utilization protein	-
E613_RS00890	150173..151600	-	1428	WP_003083784.1	GABA permease	-
E613_RS00895	151769..153262	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T75178 WP_003083773.1 NZ_CP020603:c148056-147775 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T75178 NZ_CP020603:c148056-147775 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT75178 WP_003083775.1 NZ_CP020603:c148280-148053 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT75178 NZ_CP020603:c148280-148053 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGATGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9