Detailed information of TA system TA075162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 5627..6215
• Replicon: plasmid pSSA3_1
• Accession: NZ_CP020587
• Organism: Acinetobacter nosocomialis strain SSA3

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7H2VD82
• Locus tag: B7L44_RS00030
• Protein ID: WP_023271598.1
• Coordinates: 5627..5914 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: B7L44_RS00035
• Protein ID: WP_083008170.1
• Coordinates: 5901..6215 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L44_RS00010	1700..2581	+	882	WP_005804946.1	replication initiation protein RepM	-
B7L44_RS00015	2645..3211	+	567	WP_000858429.1	plasmid replication DNA-binding protein	-
B7L44_RS00020	3441..4628	-	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
B7L44_RS00025	4694..5335	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
B7L44_RS00030	5627..5914	+	288	WP_023271598.1	BrnT family toxin	Toxin
B7L44_RS00035	5901..6215	+	315	WP_083008170.1	BrnA antitoxin family protein	Antitoxin
B7L44_RS00040	6287..6952	-	666	WP_083008171.1	DUF2290 domain-containing protein	-
B7L44_RS00045	6958..9087	-	2130	WP_083008172.1	DEAD/DEAH box helicase	-
B7L44_RS00050	9424..9648	-	225	WP_004676219.1	helix-turn-helix domain-containing protein	-
B7L44_RS00055	9798..10043	+	246	WP_004282220.1	hypothetical protein	-
B7L44_RS00060	10335..11096	-	762	WP_083008173.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39)	-	1..11400	11400

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11291.66 Da
• Isoelectric Point: 5.2216

>T75162 WP_023271598.1 NZ_CP020587:5627-5914 [Acinetobacter nosocomialis]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Nucleotide

• Length: 288 bp

>T75162 NZ_CP020587:5627-5914 [Acinetobacter nosocomialis]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGAAGATCCACTGAGGATCTCAATCCAGGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACTAAGGCGGAGCGAGATAGATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12271.87 Da
• Isoelectric Point: 6.4741

>AT75162 WP_083008170.1 NZ_CP020587:5901-6215 [Acinetobacter nosocomialis]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILREAMLEDLKNHQ

Nucleotide

• Length: 315 bp

>AT75162 NZ_CP020587:5901-6215 [Acinetobacter nosocomialis]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ATAATCAATTCTATCGCCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGCTATCAAACACGAATGAATGAAATTCTGCGAGAAGCAATGTTAGAAGATTTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A7H2VD82