Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 4520182..4520810 Replicon chromosome
Accession NZ_CP020567
Organism Kitasatospora aureofaciens strain DM-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1E7N4Q9
Locus tag B6264_RS19940 Protein ID WP_030281727.1
Coordinates 4520400..4520810 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1E7N4S0
Locus tag B6264_RS19935 Protein ID WP_030281729.1
Coordinates 4520182..4520403 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6264_RS19920 4517155..4517868 + 714 WP_030551135.1 ricin-type beta-trefoil lectin domain protein -
B6264_RS19925 4517933..4518781 - 849 WP_030551132.1 helix-turn-helix transcriptional regulator -
B6264_RS19930 4518892..4519905 + 1014 WP_141763779.1 hypothetical protein -
B6264_RS19935 4520182..4520403 + 222 WP_030281729.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
B6264_RS19940 4520400..4520810 + 411 WP_030281727.1 PIN domain-containing protein Toxin
B6264_RS19945 4520823..4521551 - 729 WP_030281725.1 sirohydrochlorin chelatase -
B6264_RS19950 4521562..4522455 - 894 WP_030281723.1 ABC transporter permease -
B6264_RS19955 4522442..4523245 - 804 WP_030281720.1 ABC transporter ATP-binding protein -
B6264_RS19960 4523416..4524579 - 1164 WP_046386237.1 aliphatic sulfonate ABC transporter substrate-binding protein -
B6264_RS19965 4524713..4524823 - 111 WP_030281715.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-43)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15155.05 Da        Isoelectric Point: 4.7483

>T75142 WP_030281727.1 NZ_CP020567:4520400-4520810 [Kitasatospora aureofaciens]
VIYLLDTSALVRLLREPKLQAAWYDAIDAESIASCYPQRAEFLYSARDGREYDEISEMFADLYPDVSVPKNAGRWIDTVQ
RRMALAGQHRSASAVDLMIAATAAHHGLTVLHDDADYRTVARCAPDLSEHSILDCA

Download         Length: 411 bp

>T75142 NZ_CP020567:4520400-4520810 [Kitasatospora aureofaciens]
GTGATCTATCTGCTCGACACCTCCGCGCTGGTCCGGCTGCTGCGCGAGCCGAAGCTTCAGGCGGCCTGGTACGACGCGAT
CGACGCGGAGTCGATCGCGTCGTGCTATCCGCAGCGTGCCGAGTTTCTCTACAGCGCGCGCGACGGCCGGGAGTACGACG
AGATCTCCGAGATGTTCGCCGACCTCTACCCGGACGTCTCAGTGCCCAAGAACGCAGGGCGCTGGATCGACACGGTGCAA
CGGCGGATGGCGCTCGCCGGGCAACATCGCAGTGCTTCCGCAGTTGACCTCATGATCGCGGCGACTGCTGCGCACCACGG
ACTCACGGTCCTCCACGACGATGCGGACTACCGCACCGTGGCGCGTTGTGCCCCGGATCTCAGTGAGCACAGCATCCTGG
ACTGCGCCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8505.56 Da        Isoelectric Point: 7.3211

>AT75142 WP_030281729.1 NZ_CP020567:4520182-4520403 [Kitasatospora aureofaciens]
MSVTQIDIDEEALERAMALAGVRTKKEAVNLALRFYAEQQERAARISRHFERARHWGAVEDAERLHRAEKDTR

Download         Length: 222 bp

>AT75142 NZ_CP020567:4520182-4520403 [Kitasatospora aureofaciens]
ATGTCCGTGACACAGATCGACATCGATGAAGAGGCGCTGGAGCGGGCGATGGCCCTCGCAGGCGTGCGGACCAAGAAGGA
AGCCGTCAACCTCGCGCTGCGCTTCTATGCGGAGCAGCAGGAACGGGCCGCCCGCATCAGTAGGCATTTCGAGCGCGCCC
GGCATTGGGGCGCAGTCGAGGACGCCGAGCGGCTGCACCGAGCCGAGAAGGACACCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E7N4Q9

Antitoxin

Source ID Structure
AlphaFold DB A0A1E7N4S0

References