Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4553353..4553961 Replicon chromosome
Accession NZ_CP020560
Organism Pseudomonas aeruginosa strain CR1

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag B7D75_RS21635 Protein ID WP_003114156.1
Coordinates 4553353..4553700 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag B7D75_RS21640 Protein ID WP_003114155.1
Coordinates 4553710..4553961 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D75_RS21605 4548792..4549004 + 213 WP_012077061.1 cysteine-rich CWC family protein -
B7D75_RS21610 4549004..4549696 + 693 WP_107114413.1 rRNA pseudouridine synthase -
B7D75_RS21615 4549832..4550875 + 1044 WP_003151256.1 L,D-transpeptidase -
B7D75_RS21620 4550955..4551692 + 738 WP_003151258.1 murein L,D-transpeptidase catalytic domain family protein -
B7D75_RS21625 4552128..4553030 + 903 WP_012077064.1 alpha/beta hydrolase -
B7D75_RS21635 4553353..4553700 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B7D75_RS21640 4553710..4553961 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B7D75_RS21645 4554175..4555158 - 984 WP_058174561.1 tyrosine-type recombinase/integrase -
B7D75_RS21650 4555158..4556450 - 1293 WP_015503465.1 hypothetical protein -
B7D75_RS21655 4556680..4557954 - 1275 WP_107114414.1 zonular occludens toxin family protein -
B7D75_RS21660 4557958..4558314 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4544166..4574690 30524


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T75135 WP_003114156.1 NZ_CP020560:c4553700-4553353 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T75135 NZ_CP020560:c4553700-4553353 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT75135 WP_003114155.1 NZ_CP020560:c4553961-4553710 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT75135 NZ_CP020560:c4553961-4553710 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References