Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 3844795..3845422 Replicon chromosome
Accession NZ_CP020559
Organism Clostridium formicaceticum strain DSM 92

Toxin (Protein)


Gene name pemK Uniprot ID A0A1D9FHI3
Locus tag CLFO_RS17885 Protein ID WP_070963824.1
Coordinates 3844795..3845145 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1D9FHE6
Locus tag CLFO_RS17890 Protein ID WP_044824195.1
Coordinates 3845147..3845422 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLFO_RS17870 3840351..3842531 - 2181 WP_070963819.1 hypothetical protein -
CLFO_RS17875 3842774..3843838 - 1065 WP_169824185.1 acyltransferase -
CLFO_RS17880 3844022..3844513 - 492 WP_070963822.1 hypothetical protein -
CLFO_RS17885 3844795..3845145 - 351 WP_070963824.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CLFO_RS17890 3845147..3845422 - 276 WP_044824195.1 ribbon-helix-helix protein, CopG family Antitoxin
CLFO_RS17895 3845575..3846747 - 1173 WP_070963826.1 alanine racemase -
CLFO_RS17900 3846922..3847551 - 630 WP_070963827.1 outer membrane lipoprotein carrier protein LolA -
CLFO_RS17905 3847682..3848050 - 369 WP_070963829.1 holo-ACP synthase -
CLFO_RS17910 3848070..3848693 - 624 WP_070963831.1 hypothetical protein -
CLFO_RS17915 3848972..3849961 + 990 WP_070963833.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12806.86 Da        Isoelectric Point: 5.1570

>T75129 WP_070963824.1 NZ_CP020559:c3845145-3844795 [Clostridium formicaceticum]
MVVKRGDIYYADLSPVIGSEQGGTRPVLVVQNDIGNRYSPTVIIAAITSQINKAKLPTHVEIAASEYGLTKDSVILLEQI
RTIDKRRLEEKIGHLDDEMMIKVSEALLISLGLVDF

Download         Length: 351 bp

>T75129 NZ_CP020559:c3845145-3844795 [Clostridium formicaceticum]
TTGGTTGTTAAAAGAGGCGACATATATTATGCAGACTTAAGTCCTGTTATAGGATCTGAGCAGGGGGGAACCAGACCCGT
TCTTGTTGTTCAAAATGATATAGGCAATCGATATAGTCCTACAGTTATTATTGCAGCTATTACTTCTCAAATCAATAAAG
CCAAGCTTCCTACCCATGTAGAAATTGCAGCCTCAGAATATGGATTAACAAAAGATTCGGTTATACTGCTAGAACAAATT
AGAACAATTGATAAAAGAAGATTAGAAGAAAAGATAGGTCATTTAGATGATGAAATGATGATAAAGGTAAGTGAGGCACT
TTTGATAAGCTTAGGGTTAGTAGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10539.18 Da        Isoelectric Point: 4.5214

>AT75129 WP_044824195.1 NZ_CP020559:c3845422-3845147 [Clostridium formicaceticum]
MADSKRIMISLPESLLKEVDYIVSMEETNRSEFVREAMKLYIREKNKVKLREKMKKGYQEMASINLTLAEVGLSLDVSSL
EDYEAEIAECE

Download         Length: 276 bp

>AT75129 NZ_CP020559:c3845422-3845147 [Clostridium formicaceticum]
ATGGCTGATTCTAAAAGGATAATGATTAGTCTTCCTGAAAGTCTTCTTAAAGAAGTAGATTATATAGTTTCTATGGAGGA
AACCAATAGAAGTGAATTTGTCAGGGAAGCCATGAAACTATATATAAGGGAAAAAAACAAAGTAAAACTAAGAGAAAAAA
TGAAAAAGGGCTATCAAGAAATGGCTTCAATCAACTTAACATTGGCAGAGGTAGGGTTAAGTCTAGATGTGTCCTCCCTA
GAAGATTATGAGGCAGAAATAGCGGAGTGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9FHI3

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9FHE6

References