Detailed information of TA system TA075128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 2700625..2701251
• Replicon: chromosome
• Accession: NZ_CP020557
• Organism: Paenibacillus larvae subsp. pulvifaciens strain SAG 10367

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: V9W2Z1
• Locus tag: B7C51_RS14360
• Protein ID: WP_023485406.1
• Coordinates: 2700901..2701251 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B7C51_RS14355
• Protein ID: WP_172423825.1
• Coordinates: 2700625..2700897 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7C51_RS26710	2695905..2696279	-	375	WP_077996683.1	IS5 family transposase	-
B7C51_RS14345	2697770..2699026	+	1257	WP_083040403.1	outer membrane lipoprotein carrier protein LolA	-
B7C51_RS14350	2699194..2700381	+	1188	WP_083040404.1	alanine racemase	-
B7C51_RS14355	2700625..2700897	+	273	WP_172423825.1	ribbon-helix-helix protein, CopG family	Antitoxin
B7C51_RS14360	2700901..2701251	+	351	WP_023485406.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7C51_RS14365	2701423..2703627	+	2205	WP_158530531.1	RNA-binding transcriptional accessory protein	-
B7C51_RS14370	2703829..2704395	-	567	WP_077997616.1	sigma-70 family RNA polymerase sigma factor	-
B7C51_RS14375	2704792..2705250	+	459	WP_083040406.1	hypothetical protein	-
B7C51_RS14380	2705842..2706069	+	228	WP_024093128.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12877.80 Da
• Isoelectric Point: 5.1393

>T75128 WP_023485406.1 NZ_CP020557:2700901-2701251 [Paenibacillus larvae subsp. pulvifaciens]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAETHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEDTMRKVNDALQVSVGLIEF

Nucleotide

• Length: 351 bp

>T75128 NZ_CP020557:2700901-2701251 [Paenibacillus larvae subsp. pulvifaciens]
TTGATTGTCAAACGTGGTGATGTTTTCTTTGCGGATCTTTCTCCTGTTGTCGGTTCCGAGCAGGGGGGAGTGAGGCCTGT
TCTTATCATTCAGAACGATATCGGGAACCGGTTTAGCCCGACGGTAATCGTGGCGGCGATTACCGCCCAAATTCAAAAAG
CCAAGCTGCCCACTCATGTGGAGATCGATGCGGAAACACACGGATTTGACCGCGATTCGGTTATCTTGCTGGAACAGATC
CGGACCATTGACAAGCAGCGGTTGACCGATAAAATTACCCATTTAGATGAAGATACCATGCGTAAAGTGAACGATGCTTT
ACAGGTAAGTGTAGGGTTGATAGAATTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10556.15 Da
• Isoelectric Point: 7.2048

>AT75128 WP_172423825.1 NZ_CP020557:2700625-2700897 [Paenibacillus larvae subsp. pulvifaciens]
MHNTKRIMISLPDHLLKEVDGIVEKENSNRSEFIRQAMRLYLLERKKRTLRESMQRGYMEMAKINLNMASEAFQAEEEAD
NTVDRLVSGV

Nucleotide

• Length: 273 bp

>AT75128 NZ_CP020557:2700625-2700897 [Paenibacillus larvae subsp. pulvifaciens]
ATGCATAATACGAAGCGTATCATGATTAGTCTTCCAGACCACCTTCTGAAGGAGGTGGACGGCATCGTCGAGAAAGAAAA
CTCGAATCGCAGCGAGTTCATTCGACAAGCCATGAGATTGTACCTGCTCGAAAGAAAGAAGAGGACCCTCCGTGAATCCA
TGCAGAGAGGCTATATGGAAATGGCGAAAATTAATTTAAATATGGCGTCCGAAGCTTTTCAAGCGGAGGAAGAAGCCGAC
AATACGGTGGACCGCTTAGTGAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A2L1TT28