Detailed information of TA system TA075127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1394793..1395388
• Replicon: chromosome
• Accession: NZ_CP020557
• Organism: Paenibacillus larvae subsp. pulvifaciens strain SAG 10367

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B7C51_RS07665
• Protein ID: WP_023483433.1
• Coordinates: 1395095..1395388 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W2E434
• Locus tag: B7C51_RS07660
• Protein ID: WP_036658190.1
• Coordinates: 1394793..1395062 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7C51_RS07640	1389806..1390420	+	615	WP_083039470.1	sigma-70 family RNA polymerase sigma factor	-
B7C51_RS07645	1390452..1392368	-	1917	WP_083039472.1	AAA family ATPase	-
B7C51_RS07650	1392628..1393914	+	1287	WP_083039474.1	MFS transporter	-
B7C51_RS07655	1394142..1394474	-	333	WP_083039476.1	hypothetical protein	-
B7C51_RS07660	1394793..1395062	+	270	WP_036658190.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B7C51_RS07665	1395095..1395388	+	294	WP_023483433.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7C51_RS07670	1395640..1395930	+	291	WP_023483432.1	metalloregulator ArsR/SmtB family transcription factor	-
B7C51_RS07675	1395927..1398426	+	2500	Protein_1518	FAD-dependent oxidoreductase	-
B7C51_RS07680	1398570..1398827	+	258	WP_077584850.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-95)

Antitoxin

• Antitoxin PemI-like (11-89)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10996.71 Da
• Isoelectric Point: 6.7162

>T75127 WP_023483433.1 NZ_CP020557:1395095-1395388 [Paenibacillus larvae subsp. pulvifaciens]
MDFNPQIGHEQAGRRPGLVVSPTSFNRVTGFIVVCPITRTVRNYPYEVPLPSDLMTEGVVLAHQVKSLDWKARKAEVREK
VPQEIIDDVLDLIHTFL

Nucleotide

• Length: 294 bp

>T75127 NZ_CP020557:1395095-1395388 [Paenibacillus larvae subsp. pulvifaciens]
ATGGATTTTAACCCGCAGATTGGACATGAACAAGCCGGAAGAAGACCAGGCCTTGTTGTCTCTCCTACATCATTCAATAG
GGTAACAGGCTTTATAGTTGTATGCCCTATTACAAGAACCGTTAGGAATTATCCATATGAAGTTCCCCTCCCTTCCGATC
TTATGACCGAAGGTGTTGTTTTGGCTCATCAAGTAAAAAGCTTGGATTGGAAGGCAAGAAAGGCAGAAGTTCGGGAAAAA
GTACCACAAGAAATTATTGACGATGTACTGGATTTAATTCACACTTTCCTATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10006.46 Da
• Isoelectric Point: 5.7379

>AT75127 WP_036658190.1 NZ_CP020557:1394793-1395062 [Paenibacillus larvae subsp. pulvifaciens]
MGKDRERMSVMSTIVSKWGNSLAIRIPSNLAESLNIKEGSEVELSIMDSTLTIKPKKRKPTLEELLAKITDGNHHEEIDF
GKPEGNEVW

Nucleotide

• Length: 270 bp

>AT75127 NZ_CP020557:1394793-1395062 [Paenibacillus larvae subsp. pulvifaciens]
ATGGGTAAAGACAGGGAAAGGATGAGTGTAATGAGCACAATTGTGAGCAAATGGGGAAACAGTCTCGCAATCCGCATTCC
AAGTAACCTGGCAGAAAGCCTAAACATAAAAGAGGGTTCTGAAGTTGAATTAAGCATCATGGATAGTACACTAACTATAA
AACCTAAAAAACGTAAGCCAACTCTTGAAGAATTACTGGCAAAAATAACAGACGGTAACCATCATGAGGAAATTGACTTT
GGAAAACCGGAGGGGAACGAGGTTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2L1TQJ8