Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 21695..22188 Replicon plasmid pZJ3920-3
Accession NZ_CP020548
Organism Escherichia coli strain ZJ3920

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag B6V57_RS28020 Protein ID WP_001230709.1
Coordinates 21934..22188 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag B6V57_RS28015 Protein ID WP_000151456.1
Coordinates 21695..21937 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6V57_RS27970 16924..17631 + 708 WP_000108794.1 hypothetical protein -
B6V57_RS27975 17651..18091 + 441 WP_023223352.1 hypothetical protein -
B6V57_RS27980 18093..18419 + 327 WP_000206697.1 hypothetical protein -
B6V57_RS27985 18679..18978 - 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
B6V57_RS27990 19480..19662 + 183 WP_001428470.1 hypothetical protein -
B6V57_RS27995 19750..20097 - 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
B6V57_RS28000 20094..20342 - 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
B6V57_RS28005 20831..21124 - 294 WP_000673428.1 hypothetical protein -
B6V57_RS28015 21695..21937 - 243 WP_000151456.1 hypothetical protein Antitoxin
B6V57_RS28020 21934..22188 - 255 WP_001230709.1 hypothetical protein Toxin
B6V57_RS28025 22185..22727 - 543 WP_001083904.1 hypothetical protein -
B6V57_RS28030 22829..23344 - 516 WP_001025393.1 J domain-containing protein -
B6V57_RS28035 23745..24074 - 330 WP_001542022.1 hypothetical protein -
B6V57_RS28045 24663..25328 + 666 WP_000532149.1 P-loop NTPase -
B6V57_RS28760 25402..25686 + 285 WP_001162371.1 hypothetical protein -
B6V57_RS28055 25704..26300 - 597 WP_000125165.1 hypothetical protein -
B6V57_RS28060 26450..26611 - 162 WP_001366886.1 type I toxin-antitoxin system Hok family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..60959 60959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T75125 WP_001230709.1 NZ_CP020548:c22188-21934 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T75125 NZ_CP020548:c22188-21934 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT75125 WP_000151456.1 NZ_CP020548:c21937-21695 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT75125 NZ_CP020548:c21937-21695 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References