Detailed information of TA system TA075124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 19750..20342
• Replicon: plasmid pZJ3920-3
• Accession: NZ_CP020548
• Organism: Escherichia coli strain ZJ3920

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: B6V57_RS28000
• Protein ID: WP_000513532.1
• Coordinates: 20094..20342 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: B6V57_RS27995
• Protein ID: WP_000681609.1
• Coordinates: 19750..20097 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6V57_RS27955	15042..15383	-	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-
B6V57_RS27960	15751..16152	+	402	WP_021575837.1	hypothetical protein	-
B6V57_RS27965	16170..16922	+	753	WP_000797966.1	conjugal transfer protein TraL	-
B6V57_RS27970	16924..17631	+	708	WP_000108794.1	hypothetical protein	-
B6V57_RS27975	17651..18091	+	441	WP_023223352.1	hypothetical protein	-
B6V57_RS27980	18093..18419	+	327	WP_000206697.1	hypothetical protein	-
B6V57_RS27985	18679..18978	-	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
B6V57_RS27990	19480..19662	+	183	WP_001428470.1	hypothetical protein	-
B6V57_RS27995	19750..20097	-	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B6V57_RS28000	20094..20342	-	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
B6V57_RS28005	20831..21124	-	294	WP_000673428.1	hypothetical protein	-
B6V57_RS28015	21695..21937	-	243	WP_000151456.1	hypothetical protein	-
B6V57_RS28020	21934..22188	-	255	WP_001230709.1	hypothetical protein	-
B6V57_RS28025	22185..22727	-	543	WP_001083904.1	hypothetical protein	-
B6V57_RS28030	22829..23344	-	516	WP_001025393.1	J domain-containing protein	-
B6V57_RS28035	23745..24074	-	330	WP_001542022.1	hypothetical protein	-
B6V57_RS28045	24663..25328	+	666	WP_000532149.1	P-loop NTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60959	60959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T75124 WP_000513532.1 NZ_CP020548:c20342-20094 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T75124 NZ_CP020548:c20342-20094 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT75124 WP_000681609.1 NZ_CP020548:c20097-19750 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT75124 NZ_CP020548:c20097-19750 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49