75123

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 116225..116718 Replicon plasmid pZJ3920-2
Accession NZ_CP020547
Organism Escherichia coli strain ZJ3920

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag B6V57_RS27820 Protein ID WP_001230709.1
Coordinates 116225..116479 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag B6V57_RS27825 Protein ID WP_000151456.1
Coordinates 116476..116718 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6V57_RS27770 111226..111501 + 276 WP_000239529.1 hypothetical protein -
B6V57_RS27775 111639..112421 - 783 WP_001372173.1 site-specific integrase -
B6V57_RS27780 112489..113097 - 609 WP_000715519.1 hypothetical protein -
B6V57_RS27785 113167..113517 - 351 WP_063100444.1 hypothetical protein -
B6V57_RS27795 114332..114661 + 330 WP_000855119.1 hypothetical protein -
B6V57_RS27810 115067..115582 + 516 WP_001025393.1 J domain-containing protein -
B6V57_RS27815 115686..116228 + 543 WP_001083904.1 hypothetical protein -
B6V57_RS27820 116225..116479 + 255 WP_001230709.1 hypothetical protein Toxin
B6V57_RS27825 116476..116718 + 243 WP_000151456.1 hypothetical protein Antitoxin
B6V57_RS27830 116855..117187 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
B6V57_RS27835 117234..118109 - 876 WP_031942271.1 class A extended-spectrum beta-lactamase CTX-M-123 -
B6V57_RS27840 118479..119176 - 698 Protein_134 IS1 family transposase -
B6V57_RS27850 119231..120400 - 1170 Protein_135 IS1380-like element ISEc9 family transposase -
B6V57_RS27855 120632..121558 + 927 WP_001290416.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / mph(A) / ant(3'')-Ia / blaCTX-M-123 - 1..127809 127809


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T75123 WP_001230709.1 NZ_CP020547:116225-116479 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T75123 NZ_CP020547:116225-116479 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT75123 WP_000151456.1 NZ_CP020547:116476-116718 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT75123 NZ_CP020547:116476-116718 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References