Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 103727..104274 Replicon plasmid pZJ3920-1
Accession NZ_CP020546
Organism Escherichia coli strain ZJ3920

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag B6V57_RS26785 Protein ID WP_000969996.1
Coordinates 103993..104274 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag B6V57_RS26780 Protein ID WP_000079941.1
Coordinates 103727..103996 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6V57_RS26730 99001..99747 + 747 WP_000205759.1 conjugal transfer pilus acetylase TraX -
B6V57_RS26735 99802..100362 + 561 WP_000139359.1 fertility inhibition protein FinO -
B6V57_RS26740 100500..100712 + 213 WP_001311693.1 hypothetical protein -
B6V57_RS26745 100955..101356 + 402 WP_001233870.1 thermonuclease family protein -
B6V57_RS26750 101401..101661 + 261 WP_000083818.1 replication regulatory protein RepA -
B6V57_RS26760 101887..101961 + 75 WP_001365705.1 RepA leader peptide Tap -
B6V57_RS26765 101954..102811 + 858 WP_000130940.1 incFII family plasmid replication initiator RepA -
B6V57_RS26780 103727..103996 + 270 WP_000079941.1 hypothetical protein Antitoxin
B6V57_RS26785 103993..104274 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B6V57_RS26790 104320..105168 + 849 WP_001057989.1 3'-5' exonuclease -
B6V57_RS26795 105354..107234 + 1881 WP_001283335.1 colicin -
B6V57_RS26800 107256..107591 - 336 WP_001080729.1 colicin 1A immunity protein -
B6V57_RS26810 107720..107977 + 258 WP_000142431.1 hypothetical protein -
B6V57_RS26815 107997..108587 - 591 WP_001350923.1 hypothetical protein -
B6V57_RS26820 108584..108844 - 261 WP_000343091.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iucA / iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB 1..133639 133639


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T75117 WP_000969996.1 NZ_CP020546:103993-104274 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T75117 NZ_CP020546:103993-104274 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT75117 WP_000079941.1 NZ_CP020546:103727-103996 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT75117 NZ_CP020546:103727-103996 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References