Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4157216..4157713 Replicon chromosome
Accession NZ_CP020543
Organism Escherichia coli C

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag B6N50_RS21275 Protein ID WP_001173343.1
Coordinates 4157216..4157389 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag B6N50_RS21280 Protein ID WP_000398619.1
Coordinates 4157417..4157713 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6N50_RS21240 (B6N50_21235) 4152546..4153121 + 576 WP_001188520.1 transcriptional regulator -
B6N50_RS21265 (B6N50_21260) 4154789..4155343 + 555 Protein_3887 dipeptide/tripeptide permease DtpC -
B6N50_RS21270 (B6N50_21265) 4155580..4157097 + 1518 WP_001295074.1 lysine--tRNA ligase -
B6N50_RS21275 (B6N50_21270) 4157216..4157389 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
B6N50_RS21280 (B6N50_21275) 4157417..4157713 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
B6N50_RS21290 (B6N50_21285) 4157940..4158212 - 273 WP_000405647.1 N-acetyltransferase -
B6N50_RS21295 (B6N50_21290) 4158224..4158454 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
B6N50_RS21300 (B6N50_21295) 4158635..4160266 + 1632 WP_047402662.1 sensor histidine kinase -
B6N50_RS21305 (B6N50_21300) 4160263..4160982 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T75079 WP_001173343.1 NZ_CP020543:c4157389-4157216 [Escherichia coli C]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T75079 NZ_CP020543:c4157389-4157216 [Escherichia coli C]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT75079 WP_000398619.1 NZ_CP020543:c4157713-4157417 [Escherichia coli C]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT75079 NZ_CP020543:c4157713-4157417 [Escherichia coli C]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References