Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3394187..3394805 Replicon chromosome
Accession NZ_CP020543
Organism Escherichia coli C

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag B6N50_RS17320 Protein ID WP_001291435.1
Coordinates 3394587..3394805 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag B6N50_RS17315 Protein ID WP_000344800.1
Coordinates 3394187..3394561 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6N50_RS17305 (B6N50_17310) 3389276..3390469 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
B6N50_RS17310 (B6N50_17315) 3390492..3393641 + 3150 WP_081508855.1 efflux RND transporter permease AcrB -
B6N50_RS17315 (B6N50_17320) 3394187..3394561 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
B6N50_RS17320 (B6N50_17325) 3394587..3394805 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
B6N50_RS17325 (B6N50_17330) 3394977..3395528 + 552 WP_000102564.1 maltose O-acetyltransferase -
B6N50_RS17330 (B6N50_17335) 3395644..3396114 + 471 WP_000136192.1 YlaC family protein -
B6N50_RS17335 (B6N50_17340) 3396278..3397828 + 1551 WP_001408474.1 cyclic-guanylate-specific phosphodiesterase PdeB -
B6N50_RS17340 (B6N50_17345) 3397870..3398223 - 354 WP_000878140.1 DUF1428 domain-containing protein -
B6N50_RS17350 (B6N50_17355) 3398602..3398913 + 312 WP_000409911.1 MGMT family protein -
B6N50_RS17355 (B6N50_17360) 3398944..3399516 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T75074 WP_001291435.1 NZ_CP020543:3394587-3394805 [Escherichia coli C]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T75074 NZ_CP020543:3394587-3394805 [Escherichia coli C]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT75074 WP_000344800.1 NZ_CP020543:3394187-3394561 [Escherichia coli C]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT75074 NZ_CP020543:3394187-3394561 [Escherichia coli C]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References