Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 882778..883432 Replicon chromosome
Accession NZ_CP020543
Organism Escherichia coli C

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag B6N50_RS04540 Protein ID WP_000244777.1
Coordinates 883025..883432 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag B6N50_RS04535 Protein ID WP_000354046.1
Coordinates 882778..883044 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6N50_RS04515 (B6N50_04515) 878747..880180 - 1434 WP_001514525.1 6-phospho-beta-glucosidase BglA -
B6N50_RS04520 (B6N50_04520) 880225..880536 + 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
B6N50_RS04525 (B6N50_04525) 880700..881359 + 660 WP_081508779.1 hemolysin III family protein -
B6N50_RS04530 (B6N50_04530) 881555..882535 - 981 WP_001514522.1 tRNA-modifying protein YgfZ -
B6N50_RS04535 (B6N50_04535) 882778..883044 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
B6N50_RS04540 (B6N50_04540) 883025..883432 + 408 WP_000244777.1 toxin CptA Toxin
B6N50_RS04545 (B6N50_04545) 883472..883993 - 522 WP_081508780.1 flavodoxin FldB -
B6N50_RS04550 (B6N50_04550) 884105..885001 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
B6N50_RS04555 (B6N50_04555) 885026..885736 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B6N50_RS04560 (B6N50_04560) 885742..887475 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T75060 WP_000244777.1 NZ_CP020543:883025-883432 [Escherichia coli C]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T75060 NZ_CP020543:883025-883432 [Escherichia coli C]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT75060 WP_000354046.1 NZ_CP020543:882778-883044 [Escherichia coli C]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT75060 NZ_CP020543:882778-883044 [Escherichia coli C]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References