Detailed information of TA system TA075055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 288863..289494
• Replicon: plasmid pSHV1
• Accession: NZ_CP020540
• Organism: Sphingobium herbicidovorans strain MH

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B6S01_RS20385
• Protein ID: WP_037466096.1
• Coordinates: 289099..289494 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A086P9A9
• Locus tag: B6S01_RS20380
• Protein ID: WP_037466097.1
• Coordinates: 288863..289096 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6S01_RS20375	285318..288341	+	3024	WP_037466099.1	conjugative relaxase	-
B6S01_RS20380	288863..289096	+	234	WP_037466097.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B6S01_RS20385	289099..289494	+	396	WP_037466096.1	type II toxin-antitoxin system VapC family toxin	Toxin
B6S01_RS20390	289727..290083	-	357	WP_037466094.1	monovalent cation/H(+) antiporter subunit G	-
B6S01_RS20395	290080..290361	-	282	WP_037466092.1	K+/H+ antiporter subunit F	-
B6S01_RS20400	290358..290846	-	489	WP_037466090.1	Na+/H+ antiporter subunit E	-
B6S01_RS20405	290843..292459	-	1617	WP_037466088.1	monovalent cation/H+ antiporter subunit D	-
B6S01_RS20410	292456..292794	-	339	WP_037466086.1	Na+/H+ antiporter subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..312167	312167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• SpoVT-AbrB domain (10-45)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14600.76 Da
• Isoelectric Point: 6.2420

>T75055 WP_037466096.1 NZ_CP020540:289099-289494 [Sphingobium herbicidovorans]
MYLLDTNILSDLVRHPQGVIGAHIAQGTTETIVTSIIVASELRFGAERRGSERLTAQLEGILKRMPVLPLEEDADRRYGA
LRAELERRGKPIGGNDMFIAAHALALDATLVTDNVREFERVPGLRVENWLR

Nucleotide

• Length: 396 bp

>T75055 NZ_CP020540:289099-289494 [Sphingobium herbicidovorans]
ATGTACCTTCTCGACACCAACATTCTTTCGGATCTGGTCCGGCATCCGCAGGGTGTAATCGGCGCGCATATCGCGCAGGG
GACTACCGAGACCATTGTCACGAGCATCATTGTCGCCAGCGAACTGCGGTTCGGGGCGGAGCGTAGGGGATCAGAGCGGT
TGACGGCGCAGCTAGAAGGGATTCTCAAACGCATGCCTGTGCTGCCGCTCGAGGAGGACGCGGATCGCCGCTATGGTGCA
CTTCGTGCGGAGCTTGAACGCCGAGGCAAACCGATCGGGGGAAATGATATGTTCATTGCCGCTCATGCCCTGGCACTCGA
CGCTACGCTCGTGACGGACAATGTCCGGGAGTTTGAGCGGGTGCCGGGATTGCGGGTCGAGAACTGGCTTCGATAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8593.78 Da
• Isoelectric Point: 4.3646

>AT75055 WP_037466097.1 NZ_CP020540:288863-289096 [Sphingobium herbicidovorans]
MNAERHVKLFKNGRSQAVRIPREFELPGEDAIMRKEGGRLIIEPAPALSLADYLATLDDIEEDFGPIDDIPADAVKF

Nucleotide

• Length: 234 bp

>AT75055 NZ_CP020540:288863-289096 [Sphingobium herbicidovorans]
ATGAACGCTGAACGTCATGTCAAACTGTTCAAAAATGGTCGCAGTCAGGCCGTCCGTATCCCGCGCGAGTTCGAGTTGCC
GGGGGAAGATGCGATTATGCGCAAGGAAGGCGGTCGGCTCATCATCGAACCCGCGCCTGCGCTGTCGCTGGCCGATTATC
TGGCTACTCTCGATGACATCGAGGAAGATTTTGGTCCAATCGACGATATTCCCGCGGATGCCGTGAAATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A086P9A9