Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 702099..702630 Replicon plasmid pMSHV
Accession NZ_CP020539
Organism Sphingobium herbicidovorans strain MH

Toxin (Protein)


Gene name relE Uniprot ID T0GNF5
Locus tag B6S01_RS17970 Protein ID WP_004212690.1
Coordinates 702099..702380 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID T0G968
Locus tag B6S01_RS17975 Protein ID WP_004212689.1
Coordinates 702367..702630 (-) Length 88 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6S01_RS17955 699658..700086 - 429 WP_021245211.1 TonB-dependent receptor -
B6S01_RS17960 700288..701016 - 729 WP_025549137.1 IS21-like element helper ATPase IstB -
B6S01_RS17965 701003..701697 - 695 Protein_687 IS21 family transposase -
B6S01_RS21435 701695..701778 - 84 Protein_688 magnesium transporter -
B6S01_RS17970 702099..702380 - 282 WP_004212690.1 type II toxin-antitoxin system YafQ family toxin Toxin
B6S01_RS17975 702367..702630 - 264 WP_004212689.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B6S01_RS17980 703014..703451 - 438 WP_007683347.1 metal-binding protein -
B6S01_RS17985 703531..704772 - 1242 WP_007683345.1 MFS transporter -
B6S01_RS17990 704766..705752 - 987 WP_004212678.1 copper resistance protein B -
B6S01_RS17995 705749..707455 - 1707 WP_021238859.1 copper resistance system multicopper oxidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..959196 959196
- flank IS/Tn - - 700288..701016 728


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-91)

Antitoxin

(4-76)


Sequences


Toxin        


Download         Length: 94 a.a.        Molecular weight: 10993.49 Da        Isoelectric Point: 7.4752

>T75053 WP_004212690.1 NZ_CP020539:c702380-702099 [Sphingobium herbicidovorans]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Download         Length: 282 bp

>T75053 NZ_CP020539:c702380-702099 [Sphingobium herbicidovorans]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9284.75 Da        Isoelectric Point: 4.5881

>AT75053 WP_004212689.1 NZ_CP020539:c702630-702367 [Sphingobium herbicidovorans]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Download         Length: 264 bp

>AT75053 NZ_CP020539:c702630-702367 [Sphingobium herbicidovorans]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATCGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB T0GNF5


Antitoxin

Source ID Structure
AlphaFold DB T0G968

References