Detailed information of TA system TA075052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 77119..77698
• Replicon: plasmid pMSHV
• Accession: NZ_CP020539
• Organism: Sphingobium herbicidovorans strain MH

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: B6S01_RS14875
• Protein ID: WP_081570479.1
• Coordinates: 77387..77698 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: I3RYM1
• Locus tag: B6S01_RS14870
• Protein ID: WP_015061620.1
• Coordinates: 77119..77376 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6S01_RS14845	73911..74627	+	717	WP_197689949.1	IS6 family transposase	-
B6S01_RS14850	74798..75034	-	237	WP_094182660.1	stable inheritance protein KleA	-
B6S01_RS14855	75184..75438	-	255	WP_015061623.1	hypothetical protein	-
B6S01_RS14860	75435..76466	-	1032	WP_031942778.1	hypothetical protein	-
B6S01_RS14865	76507..76941	-	435	WP_031942779.1	antirestriction protein	-
B6S01_RS14870	77119..77376	+	258	WP_015061620.1	transcriptional regulator	Antitoxin
B6S01_RS14875	77387..77698	+	312	WP_081570479.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B6S01_RS14885	79336..79962	+	627	Protein_75	DUF4158 domain-containing protein	-
B6S01_RS14890	79965..81344	+	1380	Protein_76	IS91 family transposase	-
B6S01_RS20870	81627..81835	-	209	Protein_77	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..959196	959196
	-	inside	IScluster/Tn	-	-	73911..81344	7433

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-102)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11039.89 Da
• Isoelectric Point: 9.3212

>T75052 WP_081570479.1 NZ_CP020539:77387-77698 [Sphingobium herbicidovorans]
IYLVSLDPTEGREQRGSRPVLVVSPAEFNAATKLPVCLPITNGGDFARRIGFAVPISGIKTTGVVRCDQPRVLDLAARTA
RKVDTLPAPILDEVLAKVATLFQ

Nucleotide

• Length: 312 bp

>T75052 NZ_CP020539:77387-77698 [Sphingobium herbicidovorans]
ATTTACCTGGTGTCGCTTGATCCCACCGAAGGCCGTGAGCAACGCGGCAGCCGGCCGGTGCTGGTCGTATCGCCGGCCGA
GTTCAACGCGGCAACGAAACTGCCGGTGTGCTTGCCGATCACGAACGGCGGCGATTTCGCCCGCCGGATCGGTTTCGCGG
TGCCGATTTCCGGCATCAAGACAACCGGCGTCGTTCGCTGCGATCAACCCCGCGTGCTCGATCTTGCAGCGCGTACCGCT
CGCAAGGTGGACACGCTCCCGGCTCCTATCCTTGATGAAGTGCTGGCCAAGGTCGCCACGCTGTTCCAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9123.48 Da
• Isoelectric Point: 4.6286

>AT75052 WP_015061620.1 NZ_CP020539:77119-77376 [Sphingobium herbicidovorans]
MHTTNLRKVGGSVMLAVPPAILDLLQLQAGATVGVAVTDGRLVVDPRPQPRYTMAELLAASDYSQPQTAEEREWVDAPAV
GRELI

Nucleotide

• Length: 258 bp

>AT75052 NZ_CP020539:77119-77376 [Sphingobium herbicidovorans]
ATGCACACAACGAATCTCCGCAAGGTCGGCGGATCGGTCATGTTGGCCGTCCCGCCGGCGATCCTCGATCTGCTGCAATT
GCAGGCCGGGGCCACGGTCGGCGTAGCCGTTACCGATGGCCGCCTGGTAGTCGATCCCAGGCCCCAGCCGCGCTACACGA
TGGCCGAGCTGCTGGCCGCCTCGGACTATTCGCAGCCACAAACTGCGGAAGAACGGGAATGGGTCGATGCCCCGGCAGTG
GGGCGTGAGCTGATATGA

Structures

Antitoxin

• AlphaFold DB: A0A086P5Y7