Detailed information of TA system TA075050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1704387..1705252
• Replicon: chromosome
• Accession: NZ_CP020538
• Organism: Sphingobium herbicidovorans strain MH

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: B6S01_RS08205
• Protein ID: WP_051908267.1
• Coordinates: 1704387..1704965 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: B6S01_RS21325
• Protein ID: WP_197053220.1
• Coordinates: 1704962..1705252 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6S01_RS21320	1700692..1701135	-	444	WP_197053219.1	hypothetical protein	-
B6S01_RS08195	1701180..1703057	-	1878	WP_197053218.1	DEAD/DEAH box helicase family protein	-
B6S01_RS08200	1703504..1704343	+	840	WP_037466148.1	DUF3883 domain-containing protein	-
B6S01_RS08205	1704387..1704965	-	579	WP_051908267.1	PIN domain-containing protein	Toxin
B6S01_RS21325	1704962..1705252	-	291	WP_197053220.1	helix-turn-helix domain-containing protein	Antitoxin
B6S01_RS08215	1705678..1706010	+	333	WP_037466146.1	polyhydroxyalkanoic acid system family protein	-
B6S01_RS08220	1706368..1708170	+	1803	WP_051908266.1	ParB N-terminal domain-containing protein	-
B6S01_RS08225	1708640..1709398	+	759	WP_051908265.1	DUF1738 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-116)

Antitoxin

• SinI-like, DNA-binding domain (27-73)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21727.87 Da
• Isoelectric Point: 4.5287

>T75050 WP_051908267.1 NZ_CP020538:c1704965-1704387 [Sphingobium herbicidovorans]
MSEIADRFVVVLDANVLYPFRVRDALLRFAEAGLFRARWSPTIMDEWTRNLLERRPDFEASIRAQMAAMERAFPESCVTG
GESLMPALILPDPDDRHVLATAIRTGAEHIVTENLKDFPDEALAPFGITAVTPDDFLSSTFELYPGDALSTLRNMRQDYQ
NPPFTPGEFIFELQRKGLPKLASMLKEHIDLL

Nucleotide

• Length: 579 bp

>T75050 NZ_CP020538:c1704965-1704387 [Sphingobium herbicidovorans]
TTGAGTGAGATCGCGGACAGATTTGTCGTCGTGCTAGACGCCAACGTCCTCTACCCCTTCCGGGTCAGGGATGCCCTGCT
TCGCTTCGCCGAGGCAGGGCTGTTCCGCGCGCGCTGGTCGCCCACGATCATGGACGAATGGACGCGTAACCTGCTCGAGA
GGCGTCCGGATTTCGAGGCGAGCATCCGGGCGCAGATGGCGGCCATGGAACGGGCATTTCCTGAAAGCTGCGTGACGGGC
GGAGAAAGCCTCATGCCAGCACTGATCCTGCCTGATCCTGATGACCGCCATGTTCTGGCGACTGCGATCCGAACAGGTGC
CGAACATATCGTCACCGAAAACCTCAAGGATTTCCCGGACGAGGCGCTGGCGCCGTTCGGCATCACGGCGGTGACGCCCG
ACGATTTTCTGAGCAGCACGTTCGAACTGTATCCAGGCGATGCCCTGTCCACCCTGCGCAACATGCGGCAGGACTACCAG
AACCCTCCCTTTACGCCGGGCGAATTCATTTTCGAACTCCAGCGCAAGGGGCTGCCCAAACTGGCTTCGATGCTTAAAGA
GCATATCGATCTGCTATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10776.48 Da
• Isoelectric Point: 9.5615

>AT75050 WP_197053220.1 NZ_CP020538:c1705252-1704962 [Sphingobium herbicidovorans]
MGQLMLDVLTHVGRGEMITLVPYGAELTTKEAADLLNVSRPFLVKLLDEGAIGYHKVGSHRRIRANELLAYKAKRDKVRR
SALTELQRLGQEFDAD

Nucleotide

• Length: 291 bp

>AT75050 NZ_CP020538:c1705252-1704962 [Sphingobium herbicidovorans]
CTGGGACAGCTCATGCTCGACGTCCTGACCCATGTCGGACGCGGCGAGATGATCACGCTCGTCCCCTATGGCGCGGAACT
TACCACGAAAGAGGCGGCAGACCTTCTCAACGTCTCTCGCCCTTTCTTGGTAAAGCTGCTGGACGAAGGCGCGATCGGCT
ACCACAAGGTCGGCTCTCACCGCAGAATACGCGCCAACGAACTCCTGGCTTACAAGGCCAAGAGGGATAAGGTTCGGCGC
AGCGCCCTGACGGAACTCCAGCGCCTTGGCCAGGAGTTTGACGCCGATTGA