Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 13432..14042 Replicon plasmid pEIB1
Accession NZ_CP020532
Organism Vibrio anguillarum strain 425

Toxin (Protein)


Gene name PumA Uniprot ID A0A3M7LLW0
Locus tag B5S57_RS00095 Protein ID WP_038529385.1
Coordinates 13746..14042 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID Q6W4R6
Locus tag B5S57_RS00090 Protein ID WP_011154656.1
Coordinates 13432..13722 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5S57_RS00070 9936..10730 + 795 WP_020977930.1 ParA family protein -
B5S57_RS00075 10727..11698 + 972 WP_011154659.1 ParB/RepB/Spo0J family partition protein -
B5S57_RS00080 11704..12087 + 384 WP_011154658.1 hypothetical protein -
B5S57_RS00085 12089..13303 + 1215 WP_020977929.1 hypothetical protein -
B5S57_RS00090 13432..13722 - 291 WP_011154656.1 putative addiction module antidote protein Antitoxin
B5S57_RS00095 13746..14042 - 297 WP_038529385.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B5S57_RS00100 14183..14401 - 219 WP_107489848.1 hypothetical protein -
B5S57_RS00105 14680..15927 + 1248 WP_013856937.1 IS91-like element ISVa3 family transposase -
B5S57_RS00110 16847..17872 + 1026 WP_020977927.1 hypothetical protein -
B5S57_RS00115 17869..18783 + 915 WP_011154651.1 helicase RepA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bauB 1..66521 66521


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(52-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11097.89 Da        Isoelectric Point: 10.7080

>T75046 WP_038529385.1 NZ_CP020532:c14042-13746 [Vibrio anguillarum]
MFTIKRTQLFDDWLKTLKDAQARGAITARVQRLTQGLSGDVKPVGSGISELRIHVGKGYRVYFKRSGKQIVVLLCGGNKK
TQNQDIERAKELVQYFDL

Download         Length: 297 bp

>T75046 NZ_CP020532:c14042-13746 [Vibrio anguillarum]
ATGTTCACTATAAAGCGCACGCAACTTTTTGATGATTGGTTGAAGACACTTAAAGATGCTCAGGCACGAGGTGCTATCAC
TGCTCGTGTTCAGCGCCTTACTCAGGGTTTGAGTGGGGATGTTAAGCCGGTTGGTTCGGGCATTAGTGAGCTTAGGATAC
ATGTCGGTAAAGGTTATCGAGTTTATTTTAAGCGTAGTGGAAAACAGATCGTTGTCTTACTTTGCGGTGGCAATAAGAAA
ACTCAGAATCAAGATATCGAACGCGCTAAAGAGTTGGTTCAATATTTTGATTTGTAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10273.59 Da        Isoelectric Point: 4.3148

>AT75046 WP_011154656.1 NZ_CP020532:c13722-13432 [Vibrio anguillarum]
MNITRLDDFDPADYLDNAESIQAYIDEAVATGDAAFISQSLGVIARAKGMNTLAEETGLSRETLYRTLSVKGNPNLKTLI
SITNALGVSVKLGSYC

Download         Length: 291 bp

>AT75046 NZ_CP020532:c13722-13432 [Vibrio anguillarum]
ATGAATATTACCAGGCTCGATGATTTTGATCCTGCTGACTATCTTGATAATGCTGAATCTATACAGGCATATATTGATGA
GGCGGTAGCAACTGGTGATGCTGCTTTTATTAGTCAATCCTTGGGTGTTATAGCCCGTGCCAAAGGTATGAATACGCTAG
CAGAGGAAACCGGTTTATCTCGTGAAACTTTATATAGAACATTAAGCGTTAAAGGGAATCCTAACCTTAAAACGCTTATC
TCTATCACTAATGCGCTTGGTGTATCTGTCAAACTCGGCTCTTACTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M7LLW0

Antitoxin

Source ID Structure
AlphaFold DB A0A3M7LK76

References