Detailed information of TA system TA075045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3332..3975
• Replicon: plasmid unnamed3
• Accession: NZ_CP020531
• Organism: Enterobacter cloacae strain 174

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: AXJ76_RS26635
• Protein ID: WP_001044770.1
• Coordinates: 3559..3975 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AXJ76_RS26630
• Protein ID: WP_001261282.1
• Coordinates: 3332..3562 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXJ76_RS27345	307..411	+	105	WP_032409716.1	hypothetical protein	-
AXJ76_RS26605	540..797	+	258	WP_000764642.1	hypothetical protein	-
AXJ76_RS26610	855..1630	-	776	Protein_2	phage integrase family protein	-
AXJ76_RS26615	1627..2370	-	744	WP_000129823.1	hypothetical protein	-
AXJ76_RS26620	2421..2771	-	351	WP_000493378.1	hypothetical protein	-
AXJ76_RS27350	2969..3375	-	407	Protein_5	hypothetical protein	-
AXJ76_RS26630	3332..3562	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AXJ76_RS26635	3559..3975	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
AXJ76_RS26640	4049..4759	+	711	WP_004152334.1	ATP-binding protein	-
AXJ76_RS26645	4793..5490	+	698	Protein_9	IS1 family transposase	-
AXJ76_RS26650	5506..5616	+	111	Protein_10	mercuric transport protein periplasmic component	-
AXJ76_RS26655	5652..6074	+	423	WP_001340589.1	organomercurial transporter MerC	-
AXJ76_RS26660	6126..7820	+	1695	WP_000105636.1	mercury(II) reductase	-
AXJ76_RS26665	7838..8200	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
AXJ76_RS26670	8197..8433	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaSHV-12	-	1..47801	47801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T75045 WP_001044770.1 NZ_CP020531:3559-3975 [Enterobacter cloacae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T75045 NZ_CP020531:3559-3975 [Enterobacter cloacae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT75045 WP_001261282.1 NZ_CP020531:3332-3562 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT75045 NZ_CP020531:3332-3562 [Enterobacter cloacae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3