Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 275442..275968 Replicon plasmid unnamed1
Accession NZ_CP020529
Organism Enterobacter cloacae strain 174

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AXJ76_RS25465 Protein ID WP_000323025.1
Coordinates 275681..275968 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AXJ76_RS25460 Protein ID WP_000534858.1
Coordinates 275442..275681 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXJ76_RS25440 271770..273008 + 1239 WP_000219087.1 IS110 family transposase -
AXJ76_RS25445 273484..274056 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
AXJ76_RS25450 274256..275179 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
AXJ76_RS25460 275442..275681 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AXJ76_RS25465 275681..275968 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AXJ76_RS25470 276040..276198 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
AXJ76_RS25475 276807..277127 - 321 WP_000332796.1 hypothetical protein -
AXJ76_RS25480 277409..277612 - 204 WP_001015183.1 hypothetical protein -
AXJ76_RS25485 277659..278009 - 351 WP_000743059.1 hypothetical protein -
AXJ76_RS25490 278069..278671 - 603 WP_012695474.1 hypothetical protein -
AXJ76_RS25495 278767..279711 - 945 WP_000778029.1 DUF5417 domain-containing protein -
AXJ76_RS27330 280222..280398 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA19 / sul1 / qnrB2 / qacE / aadA2 / aph(3')-Ia / blaSHV-12 / ant(3'')-Ia / dfrA8 / catA1 / formA / mcr-9 / aph(6)-Id - 1..400064 400064


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T75044 WP_000323025.1 NZ_CP020529:275681-275968 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T75044 NZ_CP020529:275681-275968 [Enterobacter cloacae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT75044 WP_000534858.1 NZ_CP020529:275442-275681 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT75044 NZ_CP020529:275442-275681 [Enterobacter cloacae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References