Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2463426..2464165 Replicon chromosome
Accession NZ_CP020528
Organism Enterobacter cloacae strain 174

Toxin (Protein)


Gene name tacT Uniprot ID A0A837FFP9
Locus tag AXJ76_RS12515 Protein ID WP_032609220.1
Coordinates 2463426..2463911 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag AXJ76_RS12520 Protein ID WP_003857131.1
Coordinates 2463899..2464165 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXJ76_RS12485 2459384..2459539 - 156 Protein_2377 transposase -
AXJ76_RS12490 2459640..2460443 - 804 WP_045349958.1 TIGR02391 family protein -
AXJ76_RS12495 2460465..2460944 - 480 WP_045349960.1 hypothetical protein -
AXJ76_RS12500 2461494..2462300 + 807 WP_045349962.1 hypothetical protein -
AXJ76_RS12505 2462390..2462500 - 111 Protein_2381 IS3 family transposase -
AXJ76_RS12515 2463426..2463911 - 486 WP_032609220.1 GNAT family N-acetyltransferase Toxin
AXJ76_RS12520 2463899..2464165 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
AXJ76_RS12525 2464229..2465158 - 930 WP_045349966.1 LysR family transcriptional regulator -
AXJ76_RS12530 2465288..2466676 + 1389 WP_023314162.1 MFS transporter -
AXJ76_RS12535 2466698..2467693 - 996 WP_045336282.1 DUF2891 domain-containing protein -
AXJ76_RS12540 2467703..2468689 - 987 WP_003857123.1 DUF979 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2451377..2464165 12788
- flank IS/Tn - - 2459333..2459539 206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(80-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17554.26 Da        Isoelectric Point: 9.9658

>T75033 WP_032609220.1 NZ_CP020528:c2463911-2463426 [Enterobacter cloacae]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVICKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T75033 NZ_CP020528:c2463911-2463426 [Enterobacter cloacae]
GTGGGACGTGTAACAGCGCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCATTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCACTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCCTCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT75033 WP_003857131.1 NZ_CP020528:c2464165-2463899 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT75033 NZ_CP020528:c2464165-2463899 [Enterobacter cloacae]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFP9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References