Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1085382..1086002 Replicon chromosome
Accession NZ_CP020528
Organism Enterobacter cloacae strain 174

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AXJ76_RS05365 Protein ID WP_015571250.1
Coordinates 1085382..1085600 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AXJ76_RS05370 Protein ID WP_006809850.1
Coordinates 1085628..1086002 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXJ76_RS05335 1081394..1081654 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
AXJ76_RS05340 1081657..1081797 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AXJ76_RS05345 1081794..1082504 - 711 WP_022650493.1 GNAT family N-acetyltransferase -
AXJ76_RS05350 1082606..1084066 + 1461 WP_032608667.1 PLP-dependent aminotransferase family protein -
AXJ76_RS05355 1084038..1084505 - 468 WP_022650495.1 hypothetical protein -
AXJ76_RS05360 1084622..1085173 - 552 WP_022650496.1 maltose O-acetyltransferase -
AXJ76_RS05365 1085382..1085600 - 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
AXJ76_RS05370 1085628..1086002 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AXJ76_RS05375 1086513..1089659 - 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit -
AXJ76_RS05380 1089682..1090875 - 1194 WP_045350599.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T75029 WP_015571250.1 NZ_CP020528:c1085600-1085382 [Enterobacter cloacae]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T75029 NZ_CP020528:c1085600-1085382 [Enterobacter cloacae]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT75029 WP_006809850.1 NZ_CP020528:c1086002-1085628 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT75029 NZ_CP020528:c1086002-1085628 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References