Detailed information of TA system TA075028

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 401321..402114
• Replicon: chromosome
• Accession: NZ_CP020528
• Organism: Enterobacter cloacae strain 174

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: AXJ76_RS01970
• Protein ID: WP_000854726.1
• Coordinates: 401321..401698 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: AXJ76_RS01975
• Protein ID: WP_001548930.1
• Coordinates: 401788..402114 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXJ76_RS01950	398834..399454	-	621	WP_032608268.1	DUF2238 domain-containing protein	-
AXJ76_RS01955	399700..400542	-	843	WP_001548928.1	DUF4942 domain-containing protein	-
AXJ76_RS01960	400627..400824	-	198	WP_000839269.1	DUF957 domain-containing protein	-
AXJ76_RS01965	400836..401324	-	489	WP_001547769.1	hypothetical protein	-
AXJ76_RS01970	401321..401698	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
AXJ76_RS01975	401788..402114	-	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
AXJ76_RS01980	402133..402354	-	222	WP_000691994.1	DUF987 domain-containing protein	-
AXJ76_RS01985	402363..402839	-	477	WP_000811693.1	RadC family protein	-
AXJ76_RS01990	402855..403313	-	459	WP_000211838.1	antirestriction protein	-
AXJ76_RS01995	403411..403650	-	240	WP_000194654.1	DUF905 domain-containing protein	-
AXJ76_RS02000	403727..404194	-	468	WP_045351203.1	hypothetical protein	-
AXJ76_RS02005	404218..404661	-	444	WP_045351202.1	hypothetical protein	-
AXJ76_RS02010	404661..404897	-	237	WP_001115854.1	hypothetical protein	-
AXJ76_RS02015	404934..405635	-	702	WP_001548933.1	WYL domain-containing protein	-
AXJ76_RS02020	405853..406674	-	822	WP_045351201.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	400836..410335	9499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T75028 WP_000854726.1 NZ_CP020528:c401698-401321 [Enterobacter cloacae]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T75028 NZ_CP020528:c401698-401321 [Enterobacter cloacae]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT75028 WP_001548930.1 NZ_CP020528:c402114-401788 [Enterobacter cloacae]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT75028 NZ_CP020528:c402114-401788 [Enterobacter cloacae]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7